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, JERRY G. VANDER TUIG, DALE R. ROMSOS, AND GILBERT A. LEVEILLE Departments of Food Science and Human Nutrition and Pharmacology, University, East Lansing, Michigan 48824 G. VANDER TUIG, DALE R. ROMA. LEVEILLE. Heat production and Naâ-Kâ-ATPase in lean and obese (ob/ob) . Am. J. Physiol. 238 (Endocrinol. Mab. 1): E193-E199, 1980.-The ability of young obese (ob/ob ) and lean to respond to a warm (33°C) or a cold (14°C) environment was evaluated. Heat production of both obese and lean was more than 2 times greater at 14°C than at 33°C. Because stimulation of cellular thermogenesis by cold exposure has been attributed. in part to changes in sodium pump activity, we estimated the number of Naâ-Kâ-ATPase (EC 3.6.1.3) in skelal muscle, liver, and kidneys of these from saturable E3H]ouabain to particulate tissue fractions. Neither phenotype nor environmental temperature influenced Kd values for specific ouabain . The concentration of ouabain sites in skelal muscle and liver of cold-exposed obese and lean was approximately double the number observed in warm-exposed counterparts. However, because cold-exposed obese had 43% less hindlimb muscle than warm-exposed obese , the total number of [âHlouabain sites in hindlimb muscle of cold-exposed obese was not elevated. Obese
AJP - Endocrinology and Metabolism – The American Physiological Society
Published: Mar 1, 1980
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