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Gene expression during phorbol ester-induced differentiation of cultured human megakaryoblastic cells

Gene expression during phorbol ester-induced differentiation of cultured human megakaryoblastic... Dorn, Gerald W., II, Michael G. Davis, and Drew D. D’Angelo. during phorbol ester-induced differentiation of cultured human megakaryoblastic cells. Am. J. PhysioZ. 266 (CeZZ PhysioZ. 35): C1231-C1239, 1994.Platelet protein makeup is determined during transformation of megakaryoblasts to mature s, the immediate precursor of circulating platelets. To better understand the molecular mechanisms of formation, was characterized by Northern analysis and RNA fingerprinting of cultured human CHRF-288 megakaryoblastic cells undergoing phorbol ester-stimulated megakaryocytic differentiation or serum-stimulated megakaryoblast proliferation. Protooncos c-fos and c-jun were coordinately upregulated in both proliferating and differentiating cells, whereas c-myc transcripts were upregulated during proliferation only. In contrast, mRNAs for transforming growth factor-p1 (TGFl31) and thromboxane receptors were coordinately upregulated during differentiation but differentially regulated during proliferation. RNA fingerprinting revealed multiple transcripts specific to either proliferating or differentiated cells. Three of these were identified by homology to known DNA sequence: CDw44 adhesion molecule (upregulated during differentiation), glutathione sulfhydryl peroxidase (downregulated during differentiation), and plectin cytoskeletal protein (upregulated during differentiation). Thus, although megakaryoblast proliferation and differentiation both involve DNA and protein synthesis, each growth response is characterized by a distinct pattern of . transforming growth platelets; oncos; boxane receptor; ribonucleic acid fingerprint factor; throm- gous desensitization in http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Cell Physiology The American Physiological Society

Gene expression during phorbol ester-induced differentiation of cultured human megakaryoblastic cells

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Publisher
The American Physiological Society
Copyright
Copyright © 1994 the American Physiological Society
ISSN
0363-6143
eISSN
1522-1563
Publisher site
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Abstract

Dorn, Gerald W., II, Michael G. Davis, and Drew D. D’Angelo. during phorbol ester-induced differentiation of cultured human megakaryoblastic cells. Am. J. PhysioZ. 266 (CeZZ PhysioZ. 35): C1231-C1239, 1994.Platelet protein makeup is determined during transformation of megakaryoblasts to mature s, the immediate precursor of circulating platelets. To better understand the molecular mechanisms of formation, was characterized by Northern analysis and RNA fingerprinting of cultured human CHRF-288 megakaryoblastic cells undergoing phorbol ester-stimulated megakaryocytic differentiation or serum-stimulated megakaryoblast proliferation. Protooncos c-fos and c-jun were coordinately upregulated in both proliferating and differentiating cells, whereas c-myc transcripts were upregulated during proliferation only. In contrast, mRNAs for transforming growth factor-p1 (TGFl31) and thromboxane receptors were coordinately upregulated during differentiation but differentially regulated during proliferation. RNA fingerprinting revealed multiple transcripts specific to either proliferating or differentiated cells. Three of these were identified by homology to known DNA sequence: CDw44 adhesion molecule (upregulated during differentiation), glutathione sulfhydryl peroxidase (downregulated during differentiation), and plectin cytoskeletal protein (upregulated during differentiation). Thus, although megakaryoblast proliferation and differentiation both involve DNA and protein synthesis, each growth response is characterized by a distinct pattern of . transforming growth platelets; oncos; boxane receptor; ribonucleic acid fingerprint factor; throm- gous desensitization in

Journal

AJP - Cell Physiology – The American Physiological Society

Published: May 1, 1994

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