Expression of cardiac sarcolemmal Na+-Ca2+ exchange activity in Xenopus laevis oocytes

S. Longoni , M. J. Coady , T. Ikeda , and K. D. Philipson

$MATERIALS AND METHODS toads were anesthetized on ice, a portion of the ovary was excised, and the were dissected usg watchmakerâs forceps. After dissection, the were cubated modified Barth sale [88 mM NaCl, 1 mM KCl, 2.4 mM NaHC03,0.82 mM MgS04,0.33 mM Ca(N03)2, 0.41 mM CaC12, 5 mM N-2-hydroxyethylpiperaze-Nâ-2-ethane sulfonic acid (HEPES) (pH 7.1), 0.1 mg/ml gentamyc] contag 600 U/ml collagenase (Sigma, type II; 100-300 5 ml). After 1 h at room temperature, the were extensively washed the modified Barth sale and stored at 19Â°C. After 1 expression; poly( A)âRNA; sucrose gradient day, the residual follicular layers were removed manually, and the were jected usg an apparatus similar to that described by Colman (3). Three days after jecVARIOUS ATTEMPTS at identification and isolation of the tion, the were tested for expression of Na+-Ca2+ cardiac Naâ-Ca2â r have been undertaken, none (see below). of which has been conclusive (7, 12, 21). Major hRNA was isolated from adult rabbit hearts by a phenoldrances are the absence of specific hibitors for labelg chloroform extraction procedure (11). Poly(A)+RNA was the r molecule and the need for reconstitution isolated by oligo( dT) -cellulose chromatography (Collabof each fraction to liposomes after purification steps orative$

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Expression of cardiac sarcolemmal Na+-Ca2+ exchange activity in Xenopus laevis oocytes

S. Longoni , M. J. Coady , T. Ikeda , and K. D. Philipson

Follow AJP - Cell Physiology , Volume 255: C870 The American Physiological Society – Dec 1, 1988

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Expression of cardiac sarcolemmal Na+-Ca2+ exchange activity in Xenopus laevis oocytes

S. Longoni , M. J. Coady , T. Ikeda , and K. D. Philipson

Follow AJP - Cell Physiology , Volume 255: C870 The American Physiological Society – Dec 1, 1988

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