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Evidence for age-associated reduction in acetylcholine release and smooth muscle response in the rat colon

Evidence for age-associated reduction in acetylcholine release and smooth muscle response in the... MATERIAL AND METHODS Materials ACh perchloe and atropine sulfate (both from Sigma Chemical, St. Louis, MO) were dissolved in distilled water. Tetrodotoxin (Calbiochem Behring Diagnostics, La Jolla, CA) and ti-conotoxin GVIA (Sigma) were dissolved in 0.01 N acetic acid. [Methyl-3Hjcholine chloride (Amersham International) was dissolved in pure ethanol. Ionomycin (Sigma) was dissolved in dimethyl sulfoxide. Physostigmine (eserine salicylate, Sigma) was used as an anticholinesterase, and hemicholinium-3 (Sigma) was used to prevent neural reuptake of choline. Methods CONSTIPATION IS A COMMON complaint elderly, and ic transit slows with for unclear reasons (6, 14). The effect of on ic motor activity has been examined Fischer 344 model (16). ic transit was significantly prolonged in senescent s compared with postpubertal animals. Furthermore, the response of ic smooth muscle to neural stimulation was reduced in senescent s compared with postpubertal animals. The mechanism(s) underlying the age-associated decrease in ic smooth muscle response to neural stimulation have not been examined but are thought to include 1) loss of myenteric neurons, 2) altered synthesis and/or storage of neurotransmitter(s), 3) decreased release of neurotransmitter(s), and 4) diminished smooth muscle response to neurotransmitter(s). was associated with a reduction number of neurons present myenteric plexus of the http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Gastrointestinal and Liver Physiology The American Physiological Society

Evidence for age-associated reduction in acetylcholine release and smooth muscle response in the rat colon

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Publisher
The American Physiological Society
Copyright
Copyright © 1994 the American Physiological Society
ISSN
0193-1857
eISSN
1522-1547
Publisher site
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Abstract

MATERIAL AND METHODS Materials ACh perchloe and atropine sulfate (both from Sigma Chemical, St. Louis, MO) were dissolved in distilled water. Tetrodotoxin (Calbiochem Behring Diagnostics, La Jolla, CA) and ti-conotoxin GVIA (Sigma) were dissolved in 0.01 N acetic acid. [Methyl-3Hjcholine chloride (Amersham International) was dissolved in pure ethanol. Ionomycin (Sigma) was dissolved in dimethyl sulfoxide. Physostigmine (eserine salicylate, Sigma) was used as an anticholinesterase, and hemicholinium-3 (Sigma) was used to prevent neural reuptake of choline. Methods CONSTIPATION IS A COMMON complaint elderly, and ic transit slows with for unclear reasons (6, 14). The effect of on ic motor activity has been examined Fischer 344 model (16). ic transit was significantly prolonged in senescent s compared with postpubertal animals. Furthermore, the response of ic smooth muscle to neural stimulation was reduced in senescent s compared with postpubertal animals. The mechanism(s) underlying the age-associated decrease in ic smooth muscle response to neural stimulation have not been examined but are thought to include 1) loss of myenteric neurons, 2) altered synthesis and/or storage of neurotransmitter(s), 3) decreased release of neurotransmitter(s), and 4) diminished smooth muscle response to neurotransmitter(s). was associated with a reduction number of neurons present myenteric plexus of the

Journal

AJP - Gastrointestinal and Liver Physiology – The American Physiological Society

Published: Oct 1, 1994

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