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Estimation of rat thermoregulatory ability based on body temperature response to heat

Estimation of rat thermoregulatory ability based on body temperature response to heat MATERIALSANDMETHODS Animals In experiment 1, 13-wk-old male Wistar/MK rats in heat were used (4). Wistar/MK is a well-established inbred strain and the origin of Wistar-Kyoto and SHR (Okamota-Aoki) strains. They were inbred for more than 70 generations at the Department of Zoology, Hokkaido University, Sapporo. Before our experiments, the rats were inbred for three additional generations at 24°C with illumination from 7 A.M. to 7 P.M. [light:dark (LD) l2:12]. The rats were fed standard pellets, MF (Oriental Kobo Kogyo, Tokyo), and drank tap water ad libitum. Before the heat exposure, the animals spent 3 h without food but were allowed drinking water. For experiment 2 and experiment 3, PART II, a rat colony with various thermoregulatory abilities was developed to include heatintolerant (0 min c ST < 90 min), intermediate (90 min < ST < 180 min), and heat-tolerant individuals (180 min < ST) by selective breeding. The original group of 50 male and 50 female Wistar rats from a commercial source included mostly intolerant and intermediate rats but also a few heat-tolerant rats. Before the experiment, their thermoregulatory ability in heat was improved by selective breeding for four generations. In experiment 3, PART I, male and female http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Applied Physiology The American Physiological Society

Estimation of rat thermoregulatory ability based on body temperature response to heat

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Publisher
The American Physiological Society
Copyright
Copyright © 1984 the American Physiological Society
ISSN
8750-7587
eISSN
1522-1601
Publisher site
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Abstract

MATERIALSANDMETHODS Animals In experiment 1, 13-wk-old male Wistar/MK rats in heat were used (4). Wistar/MK is a well-established inbred strain and the origin of Wistar-Kyoto and SHR (Okamota-Aoki) strains. They were inbred for more than 70 generations at the Department of Zoology, Hokkaido University, Sapporo. Before our experiments, the rats were inbred for three additional generations at 24°C with illumination from 7 A.M. to 7 P.M. [light:dark (LD) l2:12]. The rats were fed standard pellets, MF (Oriental Kobo Kogyo, Tokyo), and drank tap water ad libitum. Before the heat exposure, the animals spent 3 h without food but were allowed drinking water. For experiment 2 and experiment 3, PART II, a rat colony with various thermoregulatory abilities was developed to include heatintolerant (0 min c ST < 90 min), intermediate (90 min < ST < 180 min), and heat-tolerant individuals (180 min < ST) by selective breeding. The original group of 50 male and 50 female Wistar rats from a commercial source included mostly intolerant and intermediate rats but also a few heat-tolerant rats. Before the experiment, their thermoregulatory ability in heat was improved by selective breeding for four generations. In experiment 3, PART I, male and female

Journal

Journal of Applied Physiology – The American Physiological Society

Published: Oct 1, 1984

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