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Effects of somatostatin on intestinal calcium transport in the rat

Effects of somatostatin on intestinal calcium transport in the rat phosphorus, 0.24% magnesium, vitamin Ds (2.5 IU/ g). After an equilibration period of 7 days, the treated rats received 50 ng (0.12 nmol) of 1,2&dihydroxyvitamin D3 [1,25(OH)zD3] (kindly supplied by Dr. M. Uskokovic, Hoffman-La Roche, Nutley, NJ) subcutaneously in 95% ethanol: propanediol (50150)daily for 4 days. Control rats received vehicle alone. Animals were killed 24 h after the last injection in the nonfasting state, segments of descending colon, proximal duodenum (3 cm distal to 1st cm beyond pylorus), or ileum (distal 15 cm of small intestine) were removed for measurement of unidirectional fluxes or tissue SRIF-like immunoreactivity. In vitro flux measurements. Adjacent segments of descending colon, duodenum, or ileum from the same animal were mounted in Lucite hemichambers, with an exposed tissue area of 0.69 cm2 for colon 0.49 cm2 for the duodenum ileum. Mucosal serosal surfaces were bathed in 10 ml of bicarbonate-buffered Krebs-Ringer solution (pH 7.4) containing 1.25 mM chloride, 11 mM D-glucose, no phosphorus. Each reservoir was gassed continuously with 95% 0~~5% COa. In some experiments, mannitol (2 mM) was added 1,25dihydroxyvitamin Da; rat colon; secretion; mannitol to both reservoirs. Unidirectional transmural fluxes were measured by the technique of Ussing Zerahn (23), as modified by http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Gastrointestinal and Liver Physiology The American Physiological Society

Effects of somatostatin on intestinal calcium transport in the rat

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Publisher
The American Physiological Society
Copyright
Copyright © 1981 the American Physiological Society
ISSN
0193-1857
eISSN
1522-1547
Publisher site
See Article on Publisher Site

Abstract

phosphorus, 0.24% magnesium, vitamin Ds (2.5 IU/ g). After an equilibration period of 7 days, the treated rats received 50 ng (0.12 nmol) of 1,2&dihydroxyvitamin D3 [1,25(OH)zD3] (kindly supplied by Dr. M. Uskokovic, Hoffman-La Roche, Nutley, NJ) subcutaneously in 95% ethanol: propanediol (50150)daily for 4 days. Control rats received vehicle alone. Animals were killed 24 h after the last injection in the nonfasting state, segments of descending colon, proximal duodenum (3 cm distal to 1st cm beyond pylorus), or ileum (distal 15 cm of small intestine) were removed for measurement of unidirectional fluxes or tissue SRIF-like immunoreactivity. In vitro flux measurements. Adjacent segments of descending colon, duodenum, or ileum from the same animal were mounted in Lucite hemichambers, with an exposed tissue area of 0.69 cm2 for colon 0.49 cm2 for the duodenum ileum. Mucosal serosal surfaces were bathed in 10 ml of bicarbonate-buffered Krebs-Ringer solution (pH 7.4) containing 1.25 mM chloride, 11 mM D-glucose, no phosphorus. Each reservoir was gassed continuously with 95% 0~~5% COa. In some experiments, mannitol (2 mM) was added 1,25dihydroxyvitamin Da; rat colon; secretion; mannitol to both reservoirs. Unidirectional transmural fluxes were measured by the technique of Ussing Zerahn (23), as modified by

Journal

AJP - Gastrointestinal and Liver PhysiologyThe American Physiological Society

Published: Sep 1, 1981

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