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Direct measurement of left ventricular interstitial adenosine

Direct measurement of left ventricular interstitial adenosine 245 (Heart Circ. Physiol. 14): H327-H335, 1983.-Characteri- &ion of ’s role as a regulator of coronary blood flow requiresaccurate measurementof endogenous concentration in the (LV) interstitial compartment. Existing techniques for determining in this compartment are indirect, requiring the acceptance of major assumptionsbefore conclusionscan be drawn. We describea new technique utilizing a LV epicardial diffusion well that allows us to make rapid, direct measurementof LV interstitial concentration, avoiding many problems inherent in existing techniques. Our results show concentrations of 555pmol/ml in resting anesthetizeddogs,indicating a resting level well within the vasoactive range. Further experiments using intramyocardial bolus injections of methylene blue dye and [8-14C] indicate that the epicardial well receivesfrom a transmural distribution of LV interstitium and not from epicardial sourcesonly. The transmural interstitial is transported via small lymphatics to the epicardial surface of the heart where diffusion occurs into the epicardial well. We also examined diffusion characteristics of the parietal pericardial membrane and found that the rate constant of diffusion for this and the visceral pericardium are of the same order of magnitude, indicating that the extensively used standard pericardial superperfusate method probably underestimatescardiac interstitial concentration by 50% or more. The influence of the parietal pericardium adequately explains why http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Heart and Circulatory Physiology The American Physiological Society

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Publisher
The American Physiological Society
Copyright
Copyright © 1983 the American Physiological Society
ISSN
0363-6135
eISSN
1522-1539
Publisher site
See Article on Publisher Site

Abstract

245 (Heart Circ. Physiol. 14): H327-H335, 1983.-Characteri- &ion of ’s role as a regulator of coronary blood flow requiresaccurate measurementof endogenous concentration in the (LV) interstitial compartment. Existing techniques for determining in this compartment are indirect, requiring the acceptance of major assumptionsbefore conclusionscan be drawn. We describea new technique utilizing a LV epicardial diffusion well that allows us to make rapid, direct measurementof LV interstitial concentration, avoiding many problems inherent in existing techniques. Our results show concentrations of 555pmol/ml in resting anesthetizeddogs,indicating a resting level well within the vasoactive range. Further experiments using intramyocardial bolus injections of methylene blue dye and [8-14C] indicate that the epicardial well receivesfrom a transmural distribution of LV interstitium and not from epicardial sourcesonly. The transmural interstitial is transported via small lymphatics to the epicardial surface of the heart where diffusion occurs into the epicardial well. We also examined diffusion characteristics of the parietal pericardial membrane and found that the rate constant of diffusion for this and the visceral pericardium are of the same order of magnitude, indicating that the extensively used standard pericardial superperfusate method probably underestimatescardiac interstitial concentration by 50% or more. The influence of the parietal pericardium adequately explains why

Journal

AJP - Heart and Circulatory PhysiologyThe American Physiological Society

Published: Aug 1, 1983

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