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Changes in insulin receptor kinase with aging in rat skeletal muscle and liver

Changes in insulin receptor kinase with aging in rat skeletal muscle and liver HIDESHI KUZUYA, ATSUSHI KOSAKI, GEN OUE, IKUKO MOTOZUMI TAKAKO MAEDA, OKAMOTO, KAKEHI, MIAND HIROO been suggested that a decrease tyrose activity may be volved the mechanisms of impaired action some -resistant states (2, 3, 5, 6, 8, 13, 19, 23, 30). The present study was designed to seewhether activity alters rats. MATERIALS AND METHODS Materials [‘251]NaI and [T-~~P]ATP were purchased from New England Nuclear (Boston, MA), and wheat germ agglut (WGA)-Sepharose was obtaed from Pharmacia (Uppsala, Sweden). *Reagentsfor sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) were purchased from Bio Rad (Richmond, CA). Phenylmethylsulfonyl fluoride (PMSF), aprot, N-Xetyl-D-glucosame, bove serum album (BSA), and bove yglobul were purchased from Sigma (St. Louis, MO). Animals Male Wistar rats (n = 4) were divided to four groups by age (2, 4, 10, and 20 mo of age), body weights 291 t 21,448 t 12,562 t 23, and 654 t 30 g, respectively. Each rat was kept a cage (20 x 20 x 25 cm) free accessto a standard laboratory chow and water. After an overnight fast, the rats were killed, the livers and hdlimb muscles were quickly excised, rapidly frozen liquid nitrogen, and stored at -8OOC. Preparations of s s were prepared from http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Endocrinology and Metabolism The American Physiological Society

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Publisher
The American Physiological Society
Copyright
Copyright © 1990 the American Physiological Society
ISSN
0193-1849
eISSN
1522-1555
Publisher site
See Article on Publisher Site

Abstract

HIDESHI KUZUYA, ATSUSHI KOSAKI, GEN OUE, IKUKO MOTOZUMI TAKAKO MAEDA, OKAMOTO, KAKEHI, MIAND HIROO been suggested that a decrease tyrose activity may be volved the mechanisms of impaired action some -resistant states (2, 3, 5, 6, 8, 13, 19, 23, 30). The present study was designed to seewhether activity alters rats. MATERIALS AND METHODS Materials [‘251]NaI and [T-~~P]ATP were purchased from New England Nuclear (Boston, MA), and wheat germ agglut (WGA)-Sepharose was obtaed from Pharmacia (Uppsala, Sweden). *Reagentsfor sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) were purchased from Bio Rad (Richmond, CA). Phenylmethylsulfonyl fluoride (PMSF), aprot, N-Xetyl-D-glucosame, bove serum album (BSA), and bove yglobul were purchased from Sigma (St. Louis, MO). Animals Male Wistar rats (n = 4) were divided to four groups by age (2, 4, 10, and 20 mo of age), body weights 291 t 21,448 t 12,562 t 23, and 654 t 30 g, respectively. Each rat was kept a cage (20 x 20 x 25 cm) free accessto a standard laboratory chow and water. After an overnight fast, the rats were killed, the livers and hdlimb muscles were quickly excised, rapidly frozen liquid nitrogen, and stored at -8OOC. Preparations of s s were prepared from

Journal

AJP - Endocrinology and MetabolismThe American Physiological Society

Published: Jul 1, 1990

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