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HIDESHI KUZUYA, ATSUSHI KOSAKI, GEN OUE, IKUKO MOTOZUMI TAKAKO MAEDA, OKAMOTO, KAKEHI, MIAND HIROO been suggested that a decrease tyrose activity may be volved the mechanisms of impaired action some -resistant states (2, 3, 5, 6, 8, 13, 19, 23, 30). The present study was designed to seewhether activity alters rats. MATERIALS AND METHODS Materials [â251]NaI and [T-~~P]ATP were purchased from New England Nuclear (Boston, MA), and wheat germ agglut (WGA)-Sepharose was obtaed from Pharmacia (Uppsala, Sweden). *Reagentsfor sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) were purchased from Bio Rad (Richmond, CA). Phenylmethylsulfonyl fluoride (PMSF), aprot, N-Xetyl-D-glucosame, bove serum album (BSA), and bove yglobul were purchased from Sigma (St. Louis, MO). Animals Male Wistar rats (n = 4) were divided to four groups by age (2, 4, 10, and 20 mo of age), body weights 291 t 21,448 t 12,562 t 23, and 654 t 30 g, respectively. Each rat was kept a cage (20 x 20 x 25 cm) free accessto a standard laboratory chow and water. After an overnight fast, the rats were killed, the livers and hdlimb muscles were quickly excised, rapidly frozen liquid nitrogen, and stored at -8OOC. Preparations of s s were prepared from
AJP - Endocrinology and Metabolism – The American Physiological Society
Published: Jul 1, 1990
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