Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Amino acid stimulation of oxygen and substrate utilization by cardiac myocytes

Amino acid stimulation of oxygen and substrate utilization by cardiac myocytes The inclusion of plasma levels of the natural amino acids plus 2.5 mM glutamate and 2.5 mM malate (PAAGM) raised the oxygen consumption and glucose oxidation of isolated cardiac myocytes in phosphate buffered saline. The addition of calcium (1.25 mM) and magnesium (0.66 mM) potentiated the stimulatory effect of PAAGM on glucose oxidation and oxygen consumption, PAAGM did not alter the shape of the dose-response curve for glucose oxidation by the isolated cardiac myocyte preparation. It did increase the amount of glucose oxidation at any given media glucose concentration up to 20 mM. PAAGM also increased the rate of lactate oxidation by the isolated cardiac myocyte preparation. PAAGM did not stimulate the oxidation of octanoate unless there was glucose present in the incubation media as well. Measurements of the concentrations of free amino acids indicated higher levels in myocytes incubated in PAAGM than in myocytes incubated in phosphate buffered saline. The data suggest that substrate metabolism by the isolated cardiac myocyte preparation can be influenced by the presence of plasma constituents that would be available to the myocardium in vivo. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Gastrointestinal and Liver Physiology The American Physiological Society

Amino acid stimulation of oxygen and substrate utilization by cardiac myocytes

AJP - Gastrointestinal and Liver Physiology , Volume 235 (5): G461 – Nov 1, 1978

Loading next page...
 
/lp/the-american-physiological-society/amino-acid-stimulation-of-oxygen-and-substrate-utilization-by-cardiac-PDM18UPNND

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
The American Physiological Society
Copyright
Copyright © 1978 the American Physiological Society
ISSN
0193-1857
eISSN
1522-1547
Publisher site
See Article on Publisher Site

Abstract

The inclusion of plasma levels of the natural amino acids plus 2.5 mM glutamate and 2.5 mM malate (PAAGM) raised the oxygen consumption and glucose oxidation of isolated cardiac myocytes in phosphate buffered saline. The addition of calcium (1.25 mM) and magnesium (0.66 mM) potentiated the stimulatory effect of PAAGM on glucose oxidation and oxygen consumption, PAAGM did not alter the shape of the dose-response curve for glucose oxidation by the isolated cardiac myocyte preparation. It did increase the amount of glucose oxidation at any given media glucose concentration up to 20 mM. PAAGM also increased the rate of lactate oxidation by the isolated cardiac myocyte preparation. PAAGM did not stimulate the oxidation of octanoate unless there was glucose present in the incubation media as well. Measurements of the concentrations of free amino acids indicated higher levels in myocytes incubated in PAAGM than in myocytes incubated in phosphate buffered saline. The data suggest that substrate metabolism by the isolated cardiac myocyte preparation can be influenced by the presence of plasma constituents that would be available to the myocardium in vivo.

Journal

AJP - Gastrointestinal and Liver PhysiologyThe American Physiological Society

Published: Nov 1, 1978

There are no references for this article.