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OD,-OD, = Z (cd - E,) ([HbO,], - [HbOJJ + W/2) (cd - c,)( [HbO,l, - A&d - 4 ([HbOzL) + A& ( [Hbl) - [HbO,],) term term term term term 1 2 3 4 5 (0 + M,, H) - W,, WI INVESTIGATORS have shown that, with transmission spectrophotometry at a single appropriate wavelength, accurate measurements of arteriovenous oxygen difference (a-v 02) may be made by determining the difference in optical density between arterial and venous blood (4-7, 10). Such a-v 0, determinations can even be performed on whole blood despite its complex optical properties which result from a combination of true optical absorption and light-scattering effects (2). Under many circumstances in which continuous sampling or recirculation of arterial and venous blood is possible (e.g., isolated, perfused organ experiments; cardiopulmonary bypass; cardiac catheterization; etc.) continuous accurate a-v O2 measurements on whole, flowing blood would be desirable. Because the oximeter alone cannot determine a-v O2 directly, there is at present no commercially available instrument designed for this task. The purpose of this communication is to present our design of a solid-state dual-beam spectrophotometer which can be built âin the laboratory at relatively low cost for continuous a-v 0,
AJP - Heart and Circulatory Physiology – The American Physiological Society
Published: Apr 1, 1977
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