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A role for MAP kinase in regulating ectodomain shedding of APLP2 in corneal epithelial cells

A role for MAP kinase in regulating ectodomain shedding of APLP2 in corneal epithelial cells Abstract We previously reported an increased secretion of amyloid precursor-like protein 2 (APLP2) in the healing corneal epithelium. The present study sought to investigate signal transduction pathways involved in APLP2 shedding in vitro. APLP2 was constitutively shed and released into culture medium in SV40-immortalized human corneal epithelial cells as assessed by Western blotting, flow cytometry, and indirect immunofluorescence. Activation of protein kinase C (PKC) by phorbol 12-myristate 13-acetate (PMA) caused significant increases in APLP2 shedding. This was inhibited by staurosporine and a PKC-ε-specific, N-myristoylated peptide inhibitor. Epidermal growth factor (EGF) also induced APLP2 accumulation in culture medium. Basal APLP2 shedding as well as that induced by PMA and EGF was blocked by a mitogen-activated protein kinase (MAPK) kinase inhibitor, U-0126. Our results suggest that MAPK activity accounts for basal as well as PKC- and EGF-induced APLP2 shedding. In addition, PKC-ε may be involved in the induction of APLP2 shedding in corneal epithelial cells. amyloid precursor-like protein 2 ectodomain shedding epidermal growth factor protein kinase C mitogen-activated protein kinase Footnotes Address for reprint requests and other correspondence: F.-S. X. Yu, Schepens Eye Research Institute, 20 Staniford St., Boston, MA 02114 (E-mail: fushinyu@vision.eri.harvard.edu ). The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “ advertisement ” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. Copyright © 2001 the American Physiological Society http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Cell Physiology The American Physiological Society

A role for MAP kinase in regulating ectodomain shedding of APLP2 in corneal epithelial cells

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Publisher
The American Physiological Society
Copyright
Copyright © 2010 the American Physiological Society
ISSN
0363-6143
eISSN
1522-1563
Publisher site
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Abstract

Abstract We previously reported an increased secretion of amyloid precursor-like protein 2 (APLP2) in the healing corneal epithelium. The present study sought to investigate signal transduction pathways involved in APLP2 shedding in vitro. APLP2 was constitutively shed and released into culture medium in SV40-immortalized human corneal epithelial cells as assessed by Western blotting, flow cytometry, and indirect immunofluorescence. Activation of protein kinase C (PKC) by phorbol 12-myristate 13-acetate (PMA) caused significant increases in APLP2 shedding. This was inhibited by staurosporine and a PKC-ε-specific, N-myristoylated peptide inhibitor. Epidermal growth factor (EGF) also induced APLP2 accumulation in culture medium. Basal APLP2 shedding as well as that induced by PMA and EGF was blocked by a mitogen-activated protein kinase (MAPK) kinase inhibitor, U-0126. Our results suggest that MAPK activity accounts for basal as well as PKC- and EGF-induced APLP2 shedding. In addition, PKC-ε may be involved in the induction of APLP2 shedding in corneal epithelial cells. amyloid precursor-like protein 2 ectodomain shedding epidermal growth factor protein kinase C mitogen-activated protein kinase Footnotes Address for reprint requests and other correspondence: F.-S. X. Yu, Schepens Eye Research Institute, 20 Staniford St., Boston, MA 02114 (E-mail: fushinyu@vision.eri.harvard.edu ). The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “ advertisement ” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. Copyright © 2001 the American Physiological Society

Journal

AJP - Cell Physiology – The American Physiological Society

Published: Aug 1, 2001

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