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A kinetic model of superoxide production from single pulmonary alveolar macrophages

A kinetic model of superoxide production from single pulmonary alveolar macrophages This reaction is considered to be spontaneous and second order with respect to 02 concentration. However, if dismutase (SOD) is present, it catalyzes the dismutation reaction and effectively changes the s such that it remains second order overall but first order with respect to both 02 and SOD. Importantly, SOD increases the rate constant by two to four orders magnitude (18). The quantification 02 by PAM and other phagocytes is usually accomplished by spectrophotometrically measuring either the reduction cytochrome c, which remains soluble, or nitro blue tetrazolium (NBT), which produces a diformazan precipitate. Specificity for cytochrome c reduction has been shown by using SOD, which increased dismutation such that cytochrome c reduction was inhibited almost completely (26,31). However, NBT which is reduced to a diformazan precipitate (NBTH2) by the reaction (5) 20; + NBT+2 + 202 + NBTH2 (3) PULMONARY ALVEOLAR (PAM) protect the lungs by phagocytizing foreign debris and bacteria. This process is accompanied by the anion (02) at the surface the plasma membrane (3, 31). readily dismutates to form Hz02 and subsequently, other oxygen metabolites which have been shown to be both antibacterial and cytotoxic (21,25, 35). is produced the reduction extracellular O2 at the surface http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Cell Physiology The American Physiological Society

A kinetic model of superoxide production from single pulmonary alveolar macrophages

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Publisher
The American Physiological Society
Copyright
Copyright © 1989 the American Physiological Society
ISSN
0363-6143
eISSN
1522-1563
Publisher site
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Abstract

This reaction is considered to be spontaneous and second order with respect to 02 concentration. However, if dismutase (SOD) is present, it catalyzes the dismutation reaction and effectively changes the s such that it remains second order overall but first order with respect to both 02 and SOD. Importantly, SOD increases the rate constant by two to four orders magnitude (18). The quantification 02 by PAM and other phagocytes is usually accomplished by spectrophotometrically measuring either the reduction cytochrome c, which remains soluble, or nitro blue tetrazolium (NBT), which produces a diformazan precipitate. Specificity for cytochrome c reduction has been shown by using SOD, which increased dismutation such that cytochrome c reduction was inhibited almost completely (26,31). However, NBT which is reduced to a diformazan precipitate (NBTH2) by the reaction (5) 20; + NBT+2 + 202 + NBTH2 (3) PULMONARY ALVEOLAR (PAM) protect the lungs by phagocytizing foreign debris and bacteria. This process is accompanied by the anion (02) at the surface the plasma membrane (3, 31). readily dismutates to form Hz02 and subsequently, other oxygen metabolites which have been shown to be both antibacterial and cytotoxic (21,25, 35). is produced the reduction extracellular O2 at the surface

Journal

AJP - Cell Physiology – The American Physiological Society

Published: Feb 1, 1989

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