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Sequence Variation Of the α-Lactalbumin Gene In Holstein and Nellore Cows

Sequence Variation Of the α-Lactalbumin Gene In Holstein and Nellore Cows The α-lactalbumin is a subunit of lactose-synthase, an enzyme responsible for lactose production, a disaccharide that influences milk production. Sequence variations of bovine α -lactalbumin have been associated with differences in milk yield. This study aimed to analyze allelic frequency differences at position − 1689 (g. A > G) and + 15 (g. A > G) of the α-lactalbumin gene in Holstein (Bos taurus) and Nellore (Bos indicus) cows. Blood samples were analyzed from 34 Holstein, 104 Nellore, and 99 Dairy Nellore cows using PCR-RFLP. The different RFLP patterns were sequenced and a novel sequence variation on nucleotide − 46 was identified. An adenine at this position was designated as the A allele and a guanine was designated B allele. The frequencies of alleles A − 1689, A − 46, and A + 15 differed between Holstein and both Nellore breeds. The results show that differences in α-lactalbumin allelic variants in the 5′-flanking and the 5′-UTR region might be associated with differences in milk production between Holstein cows and cows from Nellore breeds. However, the lack of difference between Nellore and Dairy Nellore suggests that other sequence variantions that regulate milk production might be responsible for the selection of Dairy Nellore cows with superior milk production. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Animal Biotechnology Taylor & Francis

Sequence Variation Of the α-Lactalbumin Gene In Holstein and Nellore Cows

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References (9)

Publisher
Taylor & Francis
Copyright
Copyright Taylor & Francis Group, LLC
ISSN
1532-2378
eISSN
1049-5398
DOI
10.1080/10495390802058939
pmid
18607792
Publisher site
See Article on Publisher Site

Abstract

The α-lactalbumin is a subunit of lactose-synthase, an enzyme responsible for lactose production, a disaccharide that influences milk production. Sequence variations of bovine α -lactalbumin have been associated with differences in milk yield. This study aimed to analyze allelic frequency differences at position − 1689 (g. A > G) and + 15 (g. A > G) of the α-lactalbumin gene in Holstein (Bos taurus) and Nellore (Bos indicus) cows. Blood samples were analyzed from 34 Holstein, 104 Nellore, and 99 Dairy Nellore cows using PCR-RFLP. The different RFLP patterns were sequenced and a novel sequence variation on nucleotide − 46 was identified. An adenine at this position was designated as the A allele and a guanine was designated B allele. The frequencies of alleles A − 1689, A − 46, and A + 15 differed between Holstein and both Nellore breeds. The results show that differences in α-lactalbumin allelic variants in the 5′-flanking and the 5′-UTR region might be associated with differences in milk production between Holstein cows and cows from Nellore breeds. However, the lack of difference between Nellore and Dairy Nellore suggests that other sequence variantions that regulate milk production might be responsible for the selection of Dairy Nellore cows with superior milk production.

Journal

Animal BiotechnologyTaylor & Francis

Published: Jul 7, 2008

Keywords: Bovine; Holstein; α -Lactalbumin; Nellore; Sequence variation

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