Access the full text.
Sign up today, get DeepDyve free for 14 days.
D. Jackwood, S. Sommer-Wagner (2007)
Genetic characteristics of infectious bursal disease viruses from four continents.Virology, 365 2
J. Difabio, R. Li, N. Eterradossi, Toquin, Y. Gardin (1999)
European-like pathogenic infectious bursal disease viruses in Brazil.The Veterinary record, 145 7
M. Peters, T. Lin, C. Wu (2005)
Real-time RT-PCR differentiation and quantitation of infectious bursal disease virus strains using dual-labeled fluorescent probes.Journal of virological methods, 127 1
D. Bettinger, C. Mougin, B. Fouqué, B. Kantelip, J. Miguet, Michel Lab (1999)
Direct in situ reverse transcriptase-linked polymerase chain reaction with biotinylated primers for the detection of hepatitis C virus RNA in liver biopsies.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 12 3
G. Nuovo (2006)
Reverse transcriptase in situ PCR.CSH protocols, 2006 1
Pedro Junior, A. Ventura, E. Durigon (2006)
Digoxigenin-labeled probe for rabies virus nucleoprotein gene detection.Revista da Sociedade Brasileira de Medicina Tropical, 39 2
F. Ojeda, I. Škardová, M. Guarda, J. Ulloa, H. Folch (1997)
Proliferation and apoptosis in infection with infectious bursal disease virus: a flow cytometric study.Avian diseases, 41 2
Raymond Chen, S. Fuggle (1993)
In situ cDNA polymerase chain reaction. A novel technique for detecting mRNA expression.The American journal of pathology, 143 6
R. Gough, D. Alexander, M. Collins, S. Lister, W. Cox (1988)
Routine virus isolation or detection in the diagnosis of diseases in birds.Avian pathology : journal of the W.V.P.A, 17 4
Yi-Ping Li, K. Handberg, S. Kabell, M. Kusk, M. Zhang, P. Jørgensen (2007)
Relative quantification and detection of different types of infectious bursal disease virus in bursa of Fabricius and cloacal swabs using real time RT-PCR SYBR green technology.Research in veterinary science, 82 1
S. Juneja, Ramneek, D. Deka, M. Oberoi, Amarjit Singh (2008)
Molecular characterization of field isolates and vaccine strains of infectious bursal disease virus.Comparative immunology, microbiology and infectious diseases, 31 1
K. Tham, L. Young, C. Moon (1995)
Detection of infectious bursal disease virus by reverse transcription-polymerase chain reaction amplification of the virus segment A gene.Journal of virological methods, 53 2-3
G. Nuovo, P. MacConnell, A. Forde, P. Delvenne (1991)
Detection of human papillomavirus DNA in formalin-fixed tissues by in situ hybridization after amplification by polymerase chain reaction.The American journal of pathology, 139 4
X. Liu, J. Giambrone, E. Hoerr (2000)
In situ hybridization, immunohistochemistry, and in situ reverse transcription-polymerase chain reaction for detection of infectious bursal disease virus.Avian diseases, 44 1
H. Liu (2000)
Tissue print hybridisation and reverse transcriptase PCR in the detection of infectious bursal disease viruses in bursal tissues.Research in veterinary science, 68 1
Adrian Moody, Scott Sellers, Nat Bumstead (2000)
Measuring infectious bursal disease virus RNA in blood by multiplex real-time quantitative RT-PCR.Journal of virological methods, 85 1-2
M. Zhang, G. Huang, S. Qiao (2002)
Early stages of infectious bursal disease virus infection in chickens detected by in situ reverse transcriptase-polymerase chain reactionAvian Pathology, 31
G. Nuovo (2001)
Co-labeling Using In Situ PCRJournal of Histochemistry & Cytochemistry, 49
T. Berg (2000)
Acute infectious bursal disease in poultry: A reviewAvian Pathology, 29
D. Jackwood (2004)
Recent trends in the molecular diagnosis of infectious bursal disease virusesAnimal Health Research Reviews, 5
R. Kataria, A. Tiwari, G. Butchaiah, J. Kataria (1999)
Differentiation of infectious bursal disease virus strains by restriction analysis of RT-PCR-amplified VP2 gene sequences.Acta virologica, 43 4
T. Cardoso, P. Rahal, D. Pilz, Miguel Teixeira, C. Arns (2000)
Replication of classical infectious bursal disease virus in the chicken embryo related cell lineAvian Pathology, 29
M. Hamoud, P. Villegas, Susan Williams (2007)
Detection of Infectious Bursal Disease virus from Formalin-Fixed Paraffin-Embedded Tissue by Immunohistochemistry and Real-Time Reverse Transcription-Polymerase Chain ReactionJournal of Veterinary Diagnostic Investigation, 19
T. Berg, M. Gonze, G. Meulemans (1991)
Acute infectious bursal disease in poultry: Isolation and characterisation of a highly virulent strain.Avian pathology : journal of the W.V.P.A, 20 1
N. Tanimura, K. Tsukamoto, Kikuyasu Nakamura, M. Narita, M. Maeda (1995)
Association between pathogenicity of infectious bursal disease virus and viral antigen distribution detected by immunohistochemistry.Avian diseases, 39 1
Alfredo Martínez, M. Miller, K. Quinn, E. Unsworth, M. Ebina, F. Cuttitta (1995)
Non-radioactive localization of nucleic acids by direct in situ PCR and in situ RT-PCR in paraffin-embedded sections.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 43
J. Sällström, I. Zehbe, M. Alemi, E. Wilander (1993)
Pitfalls of in situ polymerase chain reaction (PCR) using direct incorporation of labelled nucleotides.Anticancer research, 13 4
T. Cardoso, R. Sousa, A. Alessi, H. Montassier, A. Pinto (1998)
A double antibody sandwich ELISA for rapid diagnosis of virus infection and to measure the humoral response against infectious bursal disease on clinical material.Avian pathology : journal of the W.V.P.A, 27 5
The presence of the very virulent (vv) Brazilian strain of infectious bursal disease virus (IBDV) was determined in the bursa of Fabricius, thymus and liver of 2-week-old broilers from a flock with a higher than expected mortality. For this purpose, a direct in situ reverse transcriptase (RT)-linked polymerase chain reaction (PCR) method was developed using specific primers for vvIBDV. Unlabelled forward and reverse biotinylated oligonucleotides were used for RT-PCR in a one-step method and the respective products were revealed by a direct enzymatic reaction. The results were compared with those obtained by standard RT-PCR using general primers for IBDV and virus isolation. The virus isolation, RT-PCR and in situ RT-PCR revealed positive results on the bursa of Fabricius in 86%, 80% and 100%, respectively. The in situ RT-PCR detected vvIBDV in all tested thymus and liver samples, whereas the standard RT-PCR detected virus in 80% and 90% of the samples, respectively. After three consecutive passages on chicken embryonated eggs, IBDV was isolated from 64% of the thymus samples and 30% of the liver samples. In the present study, no classical or antigenic variants of IBDV were detected. The developed in situ RT-PCR assay was able to detect the very virulent strain of IBDV with a higher sensitivity than the conventional RT-PCR and virus isolation.
Avian Pathology – Taylor & Francis
Published: Aug 1, 2008
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.