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A New HPLC Method for the Simultaneous Determination of Acetaminophen, Phenylephrine, Dextromethorphan and Chlorpheniramine in Pharmaceutical Formulations

Analytical Letters , Volume 41 (6): 965-976 – Apr 1, 2008

Details

Publisher
Taylor & Francis
Copyright
© 2008 Informa plc
Subject
Acetaminophen
ISSN
0003-2719
D.O.I.
10.1080/00032710801978137
Publisher site
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A New HPLC Method for the Simultaneous Determination of Acetaminophen, Phenylephrine, Dextromethorphan and Chlorpheniramine in Pharmaceutical Formulations

Abstract

Acetaminophen, phenylephrine, dextromethorphan, and chlorpheniramine are frequently associated in pharmaceutical formulations against the common cold. A new high performance liquid chromatography (HPLC) method has been developed for the simultaneous determination of these active pharmaceutical ingredients in pharmaceutical formulations. The separation and quantitation were achieved on a 25 cm underivatized silica column using a mobile phase of methanol: water (containing 6.0 g of ammonium acetate and 10 ml of triethylamine per liter, pH adjusted to 5.0 with orthophosphoric acid), 95:5%(v/v). Detection was carried out using a variable wavelength UV-vis detector at 254 nm for acetaminophen, at 220 nm for phenylephrine, and at 227 nm for dextromethorphan and chlorpheniramine. The method showed linearity for the acetaminophen, phenylephrine, dextromethorphan, and chlorpheniramine in the 162.5-650, 2.5-10, 7.5-30, and 1-4 µg/ml ranges, respectively. The intraday and interday RSDs ranged from 0.92 to 1.52%, 1.00 to 1.76%, 1.21 to 1.74% and 1.26 to 1.80% for the acetaminophen, phenylephrine, dextromethorphan, and chlorpheniramine, respectively. Compounds were eluted in a run time of less than 12 min.
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