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Validation of a Novel HPLC Method for the Determination of Raloxifene and Its Pharmacokinetics in Rat Plasma

Validation of a Novel HPLC Method for the Determination of Raloxifene and Its Pharmacokinetics in... A novel HPLC method has been developed and validated for the determination of Raloxifene in rat plasma. Samples were prepared based on a simple protein precipitation. Separation of Raloxifene in plasma was performed on a C18 column, with a mobile phase of acetonitrile–ammonium acetate. Good linearity was demonstrated in the range of 0.2–75.0 μg mL−1 (r = 0.9931). The method was used to measure the concentration and pharmacokinetics of Raloxifene in rat plasma after a single oral dose, and a linear pharmacokinetic profile of Raloxifene was found. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Chromatographia Springer Journals

Validation of a Novel HPLC Method for the Determination of Raloxifene and Its Pharmacokinetics in Rat Plasma

Chromatographia , Volume 65 (4) – Dec 20, 2006

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References (10)

Publisher
Springer Journals
Copyright
Copyright © 2006 by Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH
Subject
Chemistry; Chromatography; Proteomics; Pharmacy; Laboratory Medicine
ISSN
0009-5893
eISSN
1612-1112
DOI
10.1365/s10337-006-0123-4
Publisher site
See Article on Publisher Site

Abstract

A novel HPLC method has been developed and validated for the determination of Raloxifene in rat plasma. Samples were prepared based on a simple protein precipitation. Separation of Raloxifene in plasma was performed on a C18 column, with a mobile phase of acetonitrile–ammonium acetate. Good linearity was demonstrated in the range of 0.2–75.0 μg mL−1 (r = 0.9931). The method was used to measure the concentration and pharmacokinetics of Raloxifene in rat plasma after a single oral dose, and a linear pharmacokinetic profile of Raloxifene was found.

Journal

ChromatographiaSpringer Journals

Published: Dec 20, 2006

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