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A novel HPLC method has been developed and validated for the determination of Raloxifene in rat plasma. Samples were prepared based on a simple protein precipitation. Separation of Raloxifene in plasma was performed on a C18 column, with a mobile phase of acetonitrile–ammonium acetate. Good linearity was demonstrated in the range of 0.2–75.0 μg mL−1 (r = 0.9931). The method was used to measure the concentration and pharmacokinetics of Raloxifene in rat plasma after a single oral dose, and a linear pharmacokinetic profile of Raloxifene was found.
Chromatographia – Springer Journals
Published: Dec 20, 2006
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