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- Publisher
- Springer Journals
- Copyright
- Copyright © 1989 by Springer-Verlag
- Subject
- Life Sciences; Biotechnology; Biochemistry, general; Cell Biology; Ecology; Microbial Ecology; Microbiology
- ISSN
- 0302-8933
- eISSN
- 1432-072X
- DOI
- 10.1007/BF00409665
- Publisher site
- See Article on Publisher Site
Abstract
203 152 152 3 3 W.-Y. W. Chow J. B. Peterson A. G. Atherly Department of Genetics Iowa State University 50011 Ames IA USA Department of Botany Iowa State University 50011 Ames IA USA Institute of Molecular Biology Academica Sinica Nanking 115-29 Taipei Republic of China Abstract Tn5 was introduced into Alcaligenes eutrophus strain H1 by a suicide vector pSUP1011. Physical characterization of mutants obtained after Tn5 mutagenesis revealed a relatively high frequency of plasmid curing, or deletion of a 50 kb plasmid DNA segment. Results of Southern hybridization and chromosomal walking indicate that the same continuous stretch of plasmid DNA (designated as D region of plasmid) is deleted in four independent isolates. Moreover, the same deletion of plasmid DNA is also observed in a mitomycin C-generated mutant strain H1-4.
Journal
Archives of Microbiology – Springer Journals
Published: Aug 1, 1989