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Thidiazuron-induced shoot regeneration in pigeonpea (Cajanus cajan L.)

Thidiazuron-induced shoot regeneration in pigeonpea (Cajanus cajan L.) Thidiazuron either alone or in combination with IAA induced high frequency shoot regeneration from primary leaf segments of three pigeonpea cultivars. Transfer of the cultures to medium with reduced concentration of thidiazuron resulted in further development of the shoots. The regenerated shoots were subsequently transferred to medium supplemented with BA, IAA and gibberellic acid where 5-10% of the shoots elongated further. Rooting of shoots could be obtained on half strength MS medium supplemented with NAA. Histological studies confirmed the mode of regeneration as shoot organogenesis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Cell, Tissue and Organ Culture Springer Journals

Thidiazuron-induced shoot regeneration in pigeonpea (Cajanus cajan L.)

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References (17)

Publisher
Springer Journals
Copyright
Copyright © 1998 by Kluwer Academic Publishers
Subject
Life Sciences; Plant Sciences; Plant Physiology
ISSN
0167-6857
eISSN
1573-5044
DOI
10.1023/A:1006060318752
Publisher site
See Article on Publisher Site

Abstract

Thidiazuron either alone or in combination with IAA induced high frequency shoot regeneration from primary leaf segments of three pigeonpea cultivars. Transfer of the cultures to medium with reduced concentration of thidiazuron resulted in further development of the shoots. The regenerated shoots were subsequently transferred to medium supplemented with BA, IAA and gibberellic acid where 5-10% of the shoots elongated further. Rooting of shoots could be obtained on half strength MS medium supplemented with NAA. Histological studies confirmed the mode of regeneration as shoot organogenesis.

Journal

Plant Cell, Tissue and Organ CultureSpringer Journals

Published: Oct 8, 2004

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