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Thermal Denaturation of Bacterial Cells Examined by Differential Scanning Calorimetry

Thermal Denaturation of Bacterial Cells Examined by Differential Scanning Calorimetry Thermal stability of vegetative cells of Listeria monocytogenes, Escherichia coli and Lactobacillus plantarum was studied by counting viable fractions and determining DSC curves of their suspensions. DSC curves in the 5–99°C range showed a series of endothermic transitions between 50 and 60°C, where the heat destruction of cells occurred. Heat denaturation of DNA required a higher temperature than cell killing. Thermal death was strongly influenced by the pH, composition and NaCl content of the suspending buffer. A mathematical model developed by us enabled comparison of DSC peak temperatures and temperatures required for loss of viability. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Thermal Analysis and Calorimetry Springer Journals

Thermal Denaturation of Bacterial Cells Examined by Differential Scanning Calorimetry

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References (5)

Publisher
Springer Journals
Copyright
Copyright © 1999 by Kluwer Academic Publishers
Subject
Chemistry; Inorganic Chemistry; Physical Chemistry; Polymer Sciences; Measurement Science, Instrumentation
ISSN
1388-6150
eISSN
1572-8943
DOI
10.1023/A:1010139204401
Publisher site
See Article on Publisher Site

Abstract

Thermal stability of vegetative cells of Listeria monocytogenes, Escherichia coli and Lactobacillus plantarum was studied by counting viable fractions and determining DSC curves of their suspensions. DSC curves in the 5–99°C range showed a series of endothermic transitions between 50 and 60°C, where the heat destruction of cells occurred. Heat denaturation of DNA required a higher temperature than cell killing. Thermal death was strongly influenced by the pH, composition and NaCl content of the suspending buffer. A mathematical model developed by us enabled comparison of DSC peak temperatures and temperatures required for loss of viability.

Journal

Journal of Thermal Analysis and CalorimetrySpringer Journals

Published: Aug 1, 1999

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