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The β-mannanases elaborated by the phytopathogen Xanthomonas campestris

The β-mannanases elaborated by the phytopathogen Xanthomonas campestris 203 122 122 3 3 Robert F. H. Dekker Geoffrey P. Candy Department of Biochemistry University of Natal Pietermaritzburg Republic of South Africa Abstract The phytopathogen Xanthomonas campestris when grown on several polysaccharide substrates, including galactomannan, produced β-mannanases and carboxymethylcellulases which were present as cell wall-, or membrane-bound constituents, and also in the extracellular medium. The extracellular β-mannanases are constitutive enzymes. Cell-bound β-mannanases could be partially solubilized by Triton X-100, but this treatment could not release cell-bound cellulase. This is the first report of the existence of a cell-bound β-mannanase. Cell-free and bound β-mannanases and carboxymethylcellulases were found to be of the endoenzyme type. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Microbiology Springer Journals

The β-mannanases elaborated by the phytopathogen Xanthomonas campestris

Archives of Microbiology , Volume 122 (3) – Sep 1, 1979

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References (12)

Publisher
Springer Journals
Copyright
Copyright © 1979 by Springer-Verlag
Subject
Life Sciences; Biotechnology; Biochemistry, general; Cell Biology; Ecology; Microbial Ecology; Microbiology
ISSN
0302-8933
eISSN
1432-072X
DOI
10.1007/BF00411294
Publisher site
See Article on Publisher Site

Abstract

203 122 122 3 3 Robert F. H. Dekker Geoffrey P. Candy Department of Biochemistry University of Natal Pietermaritzburg Republic of South Africa Abstract The phytopathogen Xanthomonas campestris when grown on several polysaccharide substrates, including galactomannan, produced β-mannanases and carboxymethylcellulases which were present as cell wall-, or membrane-bound constituents, and also in the extracellular medium. The extracellular β-mannanases are constitutive enzymes. Cell-bound β-mannanases could be partially solubilized by Triton X-100, but this treatment could not release cell-bound cellulase. This is the first report of the existence of a cell-bound β-mannanase. Cell-free and bound β-mannanases and carboxymethylcellulases were found to be of the endoenzyme type.

Journal

Archives of MicrobiologySpringer Journals

Published: Sep 1, 1979

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