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203 122 122 3 3 Robert F. H. Dekker Geoffrey P. Candy Department of Biochemistry University of Natal Pietermaritzburg Republic of South Africa Abstract The phytopathogen Xanthomonas campestris when grown on several polysaccharide substrates, including galactomannan, produced β-mannanases and carboxymethylcellulases which were present as cell wall-, or membrane-bound constituents, and also in the extracellular medium. The extracellular β-mannanases are constitutive enzymes. Cell-bound β-mannanases could be partially solubilized by Triton X-100, but this treatment could not release cell-bound cellulase. This is the first report of the existence of a cell-bound β-mannanase. Cell-free and bound β-mannanases and carboxymethylcellulases were found to be of the endoenzyme type.
Archives of Microbiology – Springer Journals
Published: Sep 1, 1979
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