The Influence of Fluoride Ions upon Selected Enzymes
of Protein Metabolism in Blood Plasma of Rabbits
with Hypercholesterolemia
Ewa Birkner
&
Ewa Grucka-Mamczar
&
Sławomir Kasperczyk
&
Aleksandra Kasperczyk
&
Barbara Stawiarska-Pięta
&
Jolanta Zalejska-Fiolka
&
Beata Birkner
Received: 28 December 2007 / Accepted: 10 March 2008 /
Published online: 28 May 2008
#
Humana Press Inc. 2008
Abstract Three-month studies were performed on 18 adult rabbits of New Zealand breed
divided into three groups, with six animals in each: a control group on standard diet, a
cholesterol group receiving 500 mg of cholesterol/100 g of feed per rabbit per 24 h (CH
group), and a cholesterol+fluorine group (CH+F group) receiving 500 mg of cholesterol/
100 g of feed per rabbit per 24 h and 3 mg of F
−
/kg of body weight per 24 h. The conducted
studies proved that cholesterol in the applied dosage (500 mg cholesterol per rabbit per
24 h) has an atherogenic action. Fluoride ions administered together with a 500-mg
cholesterol atherogenic diet inhibit the atheromatosic changes in the aorta. The
concentration of plasma cholesterol was elevated in both study groups when compared to
the control group but decreased in the CH+F group when compare to the CH group. The
influence of fluoride ions has been examined upon the activity of alanine aminotransferase,
aspartate aminotransferase, and glutamate dehydrogenase (GLDH) in the plasma in the liver
of rabbits in the course of experimental hypercholesterolemia. Increase in the activity of
study enzymes has been observed in the blood plasma, which may be due to damage
occurring to hepatocytes of the animals examined (a statistically significant increase in the
activity of GLDH in the plasma). In the liver, the inhibition of activity for all examined
enzymes has been observed in the group of rabbits with hypercholesterolemia, which
testifies the disturbances in protein metabolism in examined animals. The addition of
sodium fluoride to the diet rich in cholesterol results in “removing the block” on those
Biol Trace Elem Res (2008) 124:118–128
DOI 10.1007/s12011-008-8129-4
E. Birkner
:
E. Grucka-Mamczar
:
S. Kasperczyk (*)
:
A. Kasperczyk
:
J. Zalejska-Fiolka
:
B. Birkner
Department of Biochemistry in Zabrze, Medical University of Silesia, Katowice, Poland
e-mail: skasperczyk@sum.edu.pl
B. Stawiarska-Pięta
Department of Pathology in Sosnowiec, Medical University of Silesia, Katowice, Poland