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The Fourth International Workshop on the Standardisation of Insulin Autoantibody Measurement

The Fourth International Workshop on the Standardisation of Insulin Autoantibody Measurement Diabetologia (1990) 33:638-639 Diabetologia 9 Springer-Verlag 1990 Workshop report B. Kuglin, H. Kolb, C. Greenbaum, N. K. Maclaren, ,~. Lernmark, and J. P. Palmer on Behalf of the Immunology and Diabetes Workshops and Participating Laboratories* During the course of previous insulin autoantibody (IAA) workshops [1-3] it had become apparent that fluid phase (RIA) and solid phase (ELISA) antibody detection assays gave discordant results for many sera. It was therefore decided to compare the disease association of IAA as determined by RIA vs ELISA in the Fourth IAA Workshop. For this purpose 31 participating laboratories submitted unselected sera obtained from healthy control subjects (n = 61), from patients with newly diagnosed Type 1 (insulin-dependent)diabetes mellitus (n = 30) prior to or within 24 h after the first insulin injection, and from relatives of Type I diabetic patients (n = 30). The sera were aliquoted and distributed by the Immunology of Diabetes Workshops (IDW) Laboratory in Gainesville, USA. Results from 18 fluid phase and from 10 solid phase assays could be included in the analysis. In both assays sera were tested twice, before and after competitive inhibitionwith excess insulin.Absolute counts of immunoglobulin bound iodinated insulin or absolute optical densities and/or http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Diabetologia Springer Journals

The Fourth International Workshop on the Standardisation of Insulin Autoantibody Measurement

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References (7)

Publisher
Springer Journals
Copyright
Copyright © 1990 by Springer-Verlag
Subject
Medicine & Public Health; Human Physiology; Internal Medicine; Metabolic Diseases
ISSN
0012-186X
eISSN
1432-0428
DOI
10.1007/BF00400213
Publisher site
See Article on Publisher Site

Abstract

Diabetologia (1990) 33:638-639 Diabetologia 9 Springer-Verlag 1990 Workshop report B. Kuglin, H. Kolb, C. Greenbaum, N. K. Maclaren, ,~. Lernmark, and J. P. Palmer on Behalf of the Immunology and Diabetes Workshops and Participating Laboratories* During the course of previous insulin autoantibody (IAA) workshops [1-3] it had become apparent that fluid phase (RIA) and solid phase (ELISA) antibody detection assays gave discordant results for many sera. It was therefore decided to compare the disease association of IAA as determined by RIA vs ELISA in the Fourth IAA Workshop. For this purpose 31 participating laboratories submitted unselected sera obtained from healthy control subjects (n = 61), from patients with newly diagnosed Type 1 (insulin-dependent)diabetes mellitus (n = 30) prior to or within 24 h after the first insulin injection, and from relatives of Type I diabetic patients (n = 30). The sera were aliquoted and distributed by the Immunology of Diabetes Workshops (IDW) Laboratory in Gainesville, USA. Results from 18 fluid phase and from 10 solid phase assays could be included in the analysis. In both assays sera were tested twice, before and after competitive inhibitionwith excess insulin.Absolute counts of immunoglobulin bound iodinated insulin or absolute optical densities and/or

Journal

Diabetologia – Springer Journals

Published: Oct 1, 1990

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