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The acid tolerance response of Bacillus cereus ATCC14579 is dependent on culture pH, growth rate and intracellular pH

The acid tolerance response of Bacillus cereus ATCC14579 is dependent on culture pH, growth rate... The food pathogen Bacillus cereus is likely to encounter acidic environments (i) in food when organic acids are added for preservation purposes, and (ii) during the stomachal transit of aliments. In order to characterise the acid stress response of B. cereus ATCC14579, cells were grown in chemostat at different pH values (pH o from 9.0 to 5.5) and different growth rates ( μ from 0.1 to 0.8 h −1 ), and were submitted to acid shock at pH 4.0. Cells grown at low pH o were adapted to acid media and induced a significant acid tolerance response (ATR). The ATR induced was modulated by both pH o and μ , and the μ effect was more marked at pH o 5.5. Intracellular pH (pH i ) was affected by both pH o and μ . At a pH o above 6, the pH i decreased with the decrease of pH o and the increase of μ . At pH o 5.5, pH i was higher compared to pH o 6.0, suggesting that mechanisms of pH i homeostasis were induced. The acid survival of B. cereus required protein neo-synthesis and the capacity of cells to maintain their pH i and ΔpH (pH i - pH o ). Haemolysin BL and non-haemolytic enterotoxin production were both influenced by pH o and μ . http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Microbiology Springer Journals

The acid tolerance response of Bacillus cereus ATCC14579 is dependent on culture pH, growth rate and intracellular pH

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References (32)

Publisher
Springer Journals
Copyright
Copyright © 2006 by Springer-Verlag
Subject
Life Sciences; Microbiology ; Microbial Ecology; Ecology; Cell Biology; Biochemistry, general; Biotechnology
ISSN
0302-8933
eISSN
1432-072X
DOI
10.1007/s00203-006-0137-1
pmid
16906407
Publisher site
See Article on Publisher Site

Abstract

The food pathogen Bacillus cereus is likely to encounter acidic environments (i) in food when organic acids are added for preservation purposes, and (ii) during the stomachal transit of aliments. In order to characterise the acid stress response of B. cereus ATCC14579, cells were grown in chemostat at different pH values (pH o from 9.0 to 5.5) and different growth rates ( μ from 0.1 to 0.8 h −1 ), and were submitted to acid shock at pH 4.0. Cells grown at low pH o were adapted to acid media and induced a significant acid tolerance response (ATR). The ATR induced was modulated by both pH o and μ , and the μ effect was more marked at pH o 5.5. Intracellular pH (pH i ) was affected by both pH o and μ . At a pH o above 6, the pH i decreased with the decrease of pH o and the increase of μ . At pH o 5.5, pH i was higher compared to pH o 6.0, suggesting that mechanisms of pH i homeostasis were induced. The acid survival of B. cereus required protein neo-synthesis and the capacity of cells to maintain their pH i and ΔpH (pH i - pH o ). Haemolysin BL and non-haemolytic enterotoxin production were both influenced by pH o and μ .

Journal

Archives of Microbiology – Springer Journals

Published: Sep 1, 2006

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