Access the full text.
Sign up today, get DeepDyve free for 14 days.
Loading next page...
/lp/springer-journals/synthesis-of-galacto-oligosaccharides-by-a-cloned-galactosidase-from-2mEYzwQCgd
References (17)
-
C. Aslanidis, Kurt Schmid, Rüdiger Schmitt (1989)
Nucleotide sequences and operon structure of plasmid-borne genes mediating uptake and utilization of raffinose in Escherichia coliJournal of Bacteriology, 171
-
N. Overbeeke, A. Fellinger, M. Toonen, Dick Wassenaar, C. Verrips (1989)
Cloning and nucleotide sequence of the α-galactosidase cDNA from Cyamopsis tetragonoloba (guar)Plant Molecular Biology, 13
-
J. Sedmak, S. Grossberg (1977)
A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250.Analytical biochemistry, 79 1-2
-
H. Shibuya, H. Nagasaki, S. Kaneko, S. Yoshida, G. Park, I. Kusakabe, Hideyuki Kobayashi (1998)
Cloning and High-Level Expression of α-Galactosidase cDNA from Penicillium purpurogenumApplied and Environmental Microbiology, 64
-
M. Hung, B. Lee (1998)
Cloning and expression of β-galactosidase gene from Bifidobacterium infantis into Escherichia coliBiotechnology Letters, 20
-
N. Nunoura, K. Ohdan, Keiko Tanaka, H. Tamaki, T. Yano, M. Inui, H. Yukawa, Kenji Yamamoto, H. Kumagai (1996)
Cloning and Nucleotide Sequence of the β-d-Glucosidase Gene from Bifidobacterium breve clb, and Expression of β-d-Glucosidase Activity in Escherichia coliBioscience, Biotechnology, and Biochemistry, 60
-
A. Zhu, J. Goldstein (1994)
Cloning and functional expression of a cDNA encoding coffee bean α-galactosidaseGene, 140
-
G. O’Hara, I. Riley, A. Glenn, M. Dilworth (1985)
The Ammonium Permease of Rhizobium leguminosarum MNF3841Microbiology, 131
-
K. Laere, M. Bosveld, H. Schols, G. Beldman, A. Voragen (1997)
Fermentative degradation of plant cell wall derived oligosaccharides by intestinal bacteria. -
Denis Roy, Pierre Ward, Guy Champagne (1996)
Differentiation of bifidobacteria by use of pulsed-field gel electrophoresis and polymerase chain reaction.International journal of food microbiology, 29 1
-
I. Herder, Ana Rosell, C. Zuilen, P. Punt, C. Hondel (1992)
Cloning and expression of a member of the Aspergillus niger gene family encoding α-galactosidaseMolecular and General Genetics MGG, 233
-
J. Sambrook, E. Fritsch, T. Maniatis (2001)
Molecular Cloning: A Laboratory Manual -
M. King, D. Yernool, D. Eveleigh, B. Chassy (1998)
Thermostable α‐galactosidase from Thermotoga neapolitana: cloning, sequencing and expressionFems Microbiology Letters, 163
-
B. Henrissat, A. Bairoch (1996)
Updating the sequence-based classification of glycosyl hydrolases.The Biochemical journal, 316 ( Pt 2)
-
S. Leder, W. Hartmeier, S. Marx (1999)
α-Galactosidase of Bifidobacterium adolescentis DSM 20083Current Microbiology, 38
-
(1998)
Trends Food Sci . Technol -
P. Gerhardt (1994)
Methods for general and molecular bacteriology
- Publisher
- Springer Journals
- Copyright
- Copyright © 1999 by Kluwer Academic Publishers
- Subject
- Life Sciences; Biotechnology; Organic Chemistry; Biochemistry, general; Microbiology; Animal Anatomy / Morphology / Histology
- ISSN
- 0141-5492
- eISSN
- 1573-6776
- DOI
- 10.1023/A:1005542521708
- Publisher site
- See Article on Publisher Site
Abstract
A genomic library of Bifidobacterium adolescentis was constructed in Escherichia coli and a gene encoding an α-galactosidase was isolated. The identified open reading frame showed high similarity and identity with bacterial α-galactosidases, which belong to Family 36 of the glycosyl hydrolases. For the purification of the enzyme from the medium a single chromatography step was sufficient. The yield of the recombinant enzyme was 100 times higher than from B. adolescentis itself. In addition to hydrolytic activity the α-galactosidase showed transglycosylation activity and can be used for the production of α-galacto-oligosaccharides.
Journal
Biotechnology Letters – Springer Journals
Published: Oct 1, 2004