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Study of biologically relevant physical-chemical properties of bile salts by reverse-phase liquid chromatography

Study of biologically relevant physical-chemical properties of bile salts by reverse-phase liquid... The study of chromatographic behaviour of bile salts with reverse-phase HPLC columns (C18 and C8) and buffered water — methanol eluent systems indicated that the octanol-water partition coefficient and the micellar cholesterol-solubilizing capacity are linearly correlated with the chromatographic mobilities if eluents with pH>7 and 0.154 M saline concentration are used (correlation coefficients 0.98 and 0.86 respectively). The critical micellar concentration was poorly correlated with the chromatographic mobility under the various conditions tested. The pKa values of bile salts were determined by the changes of mobility with the pH of eluent. Values in the range 4.92–5.06 and 4.27–4.47 were found respectively for unconjugated and glycineconjugated bile salt, in agreement with previous estimates. Values in the range 0.74–1.02 represent the first direct evaluation for taurine-conjugates. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Chromatographia Springer Journals

Study of biologically relevant physical-chemical properties of bile salts by reverse-phase liquid chromatography

Cantafora, A.; Biase, A.; Angelico, M.; Alvaro, D.
Chromatographia , Volume 24 (1) – Dec 1, 1987
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References (17)

Publisher
Springer Journals
Copyright
Copyright © 1987 by Friedr. Vieweg & Sohn Verlagsgesellschaft mbH
Subject
Chemistry; Analytical Chemistry; Organic Chemistry; Pharmacy; Biochemistry, general; Plant Sciences; Measurement Science, Instrumentation
ISSN
0009-5893
eISSN
1612-1112
DOI
10.1007/BF02688489
Publisher site
See Article on Publisher Site

Abstract

The study of chromatographic behaviour of bile salts with reverse-phase HPLC columns (C18 and C8) and buffered water — methanol eluent systems indicated that the octanol-water partition coefficient and the micellar cholesterol-solubilizing capacity are linearly correlated with the chromatographic mobilities if eluents with pH>7 and 0.154 M saline concentration are used (correlation coefficients 0.98 and 0.86 respectively). The critical micellar concentration was poorly correlated with the chromatographic mobility under the various conditions tested. The pKa values of bile salts were determined by the changes of mobility with the pH of eluent. Values in the range 4.92–5.06 and 4.27–4.47 were found respectively for unconjugated and glycineconjugated bile salt, in agreement with previous estimates. Values in the range 0.74–1.02 represent the first direct evaluation for taurine-conjugates.

Journal

Chromatographia – Springer Journals

Published: Dec 1, 1987

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