Relationships among Iron, Protein Oxidation
and Lipid Peroxidation Levels in Rats
with Alcohol-induced Acute Pancreatitis
Ali Riza Kiziler
&
Birsen Aydemir
&
Tevfik Gulyasar
&
Ethem Unal
&
Pembegul Gunes
Received: 6 November 2007 / Accepted: 10 March 2008 /
Published online: 12 April 2008
#
Humana Press Inc. 2008
Abstract It has been previously shown that alcohol induces the damage of pancreatic
parenchyma tissue, but the mechanism of this damage is still poorly understood. Assuming
that oxygen radical damage may be the involved, we measured markers of oxidative
damage in pancreatic tissue, blood serum, plasma, and whole blood of rats with early-stage
alcohol-induced acute pancreatitis. Thirty-eight male Wistar rats were divided into three
groups: the control group (group 1), the acute pancreatitis group 1 day (group 2), and
3 days (group 3) after the injection of ethyl alcohol into the common biliary duct,
respectively. The levels of Fe in tissue and serum, whole blood viscosity, plasma viscosity,
fibrinogen and homocysteine (Hcy) levels, erythrocyte and plasma malondialdehyde
(MDA), and tissue and plasma protein carbonyl levels were found to be significantly
higher in groups 2 and 3 than in group 1. However, the levels of reduced glutathione (GSH)
in tissue and erythrocytes were significantly lower in groups 2 and 3 than in group 1. These
results suggest that elevated Fe levels in serum and pancreatic tissue in rats with early-stage
alcohol-induced acute pancreatitis is associated with various hemorheological changes and
with oxidative damage of the pancreas.
Keywords Iron
.
Homocysteine
.
Malondialdehyde
.
Protein carbonyls
.
Viscosity
.
Acute pancreatitis
Biol Trace Elem Res (2008) 124:135–143
DOI 10.1007/s12011-008-8127-6
A. R. Kiziler
:
B. Aydemir
Department of Biophysics, Cerrahpasa Faculty of Medicine, Istanbul University, 34098 Istanbul, Turkey
T. Gulyasar
Department of Biophysics, Medical Faculty, Trakya University, 22030 Edirne, Turkey
E. Unal
Department of General Surgery, Haydarpasa Numune Training and Research Hospital, 34668 Istanbul,
Turkey
P. Gunes
Department of Pathology, Haydarpasa Numune Training and Research Hospital, 34668 Istanbul, Turkey
A. R. Kiziler (*)
Ramazanoglu Mah, Kaynarca Cad, No. 74, Botas Lojmanlari, Pendik, 34892 Istanbul, Turkey
e-mail: ark@istanbul.edu.tr