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Pyrococcus abyssi sp. nov., a new hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent

Pyrococcus abyssi sp. nov., a new hyperthermophilic archaeon isolated from a deep-sea... 203 160 160 5 5 Gaël Erauso Anna-Louise Reysenbach Anne Godfroy Jean-Roch Meunier Byron Crump Frédéric Partensky John A. Baross Viggo Marteinsson Georges Barbier Norman R. Pace Daniel Prieur Station Biologique CNRS UPR 4601 F-29680 Roscoff France Department of Biology Indiana University 47405 Bloomington IN USA IFREMER Centre de Brest: Environment Profond F-29280 Plouzané France Unité des entérobactéries Institut Pasteur 28 rue du docteur Roux F-75724 Paris Cédex France School of Oceanography University of Washington 98195 Seattle WA USA Abstract A novel, hyperthermophilic, anaerobic, sulfurmetabolizing archaeon was isolated from a fluid sample from recently discovered hydrothermal vents in the North Fiji basin (SW Pacific), at 2000 m depth. The new organism, strain GE5, is a gram-negative, highly motile coccus. It grows between 67° and 102°C under atmospheric pressure, with an optimum at 96°C (doubling time 33 min). The upper growth temperature is extended by at least 3°C when cells are cultivated under in situ hydrostatic pressures (20 MPa). Strain GE5 is an obligate heterotroph, fermenting peptides, or mixtures of amino acids to acetate, isovalerate, isobutyrate, propionate, H 2 and CO 2 . Hydrogen inhibits growth unless sulfur is present. In the presence of sulfur, H 2 S is then produced. Phylogenetic analyses of the 16 S rRNA sequence of strain GE5 places the new isolate within the Thermococcales . By its high growth temperature and physiological features the new isolate ressembles Pyrococcus sp. However it deffers by a 7% mol upper G+C-content and shows low level of DNA similarity with the two previously described species. Based on these differences the description of strain GE5 as a new species Pyrococcus abyssi (CNCM I-1302) is proposed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Microbiology Springer Journals

Pyrococcus abyssi sp. nov., a new hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent

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References (64)

Publisher
Springer Journals
Copyright
Copyright © 1993 by Springer-Verlag
Subject
Life Sciences; Biotechnology; Biochemistry, general; Cell Biology; Ecology; Microbial Ecology; Microbiology
ISSN
0302-8933
eISSN
1432-072X
DOI
10.1007/BF00252219
Publisher site
See Article on Publisher Site

Abstract

203 160 160 5 5 Gaël Erauso Anna-Louise Reysenbach Anne Godfroy Jean-Roch Meunier Byron Crump Frédéric Partensky John A. Baross Viggo Marteinsson Georges Barbier Norman R. Pace Daniel Prieur Station Biologique CNRS UPR 4601 F-29680 Roscoff France Department of Biology Indiana University 47405 Bloomington IN USA IFREMER Centre de Brest: Environment Profond F-29280 Plouzané France Unité des entérobactéries Institut Pasteur 28 rue du docteur Roux F-75724 Paris Cédex France School of Oceanography University of Washington 98195 Seattle WA USA Abstract A novel, hyperthermophilic, anaerobic, sulfurmetabolizing archaeon was isolated from a fluid sample from recently discovered hydrothermal vents in the North Fiji basin (SW Pacific), at 2000 m depth. The new organism, strain GE5, is a gram-negative, highly motile coccus. It grows between 67° and 102°C under atmospheric pressure, with an optimum at 96°C (doubling time 33 min). The upper growth temperature is extended by at least 3°C when cells are cultivated under in situ hydrostatic pressures (20 MPa). Strain GE5 is an obligate heterotroph, fermenting peptides, or mixtures of amino acids to acetate, isovalerate, isobutyrate, propionate, H 2 and CO 2 . Hydrogen inhibits growth unless sulfur is present. In the presence of sulfur, H 2 S is then produced. Phylogenetic analyses of the 16 S rRNA sequence of strain GE5 places the new isolate within the Thermococcales . By its high growth temperature and physiological features the new isolate ressembles Pyrococcus sp. However it deffers by a 7% mol upper G+C-content and shows low level of DNA similarity with the two previously described species. Based on these differences the description of strain GE5 as a new species Pyrococcus abyssi (CNCM I-1302) is proposed.

Journal

Archives of Microbiology – Springer Journals

Published: Nov 1, 1993

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