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Purification and properties of cytochrome c (550; Hemispora stellata )

Purification and properties of cytochrome c (550; Hemispora stellata ) 203 88 88 1 1 J. Francisco T. Sala Justino Burgos Facultad de Veterinaria Cátedra de Bioquímica y Tecnología de los Alimentos León Spain Summary 1. A water soluble cytochrome c from Hemispora stellata has been obtained in a highly purified state by a series of steps involving (NH 4 ) 2 SO 4 precipitation and DEAE chromatography. 2. Absorption spectrum of the highly purified cytochrome c (550; H. stellata ) in the reduced state shows maxima at 550, 520, 416, 357–362 nm and 310–320 nm. It has a symmetrical α band and ratios of α red. /prot. ox and Soret red /α red. are 1 and 6.4 respectively. The purified cytochrome has a mesoheme prosthetic group, its molecular weight is about 17500, its isoelectric point is at pH higher than 8.6 and its É o is+0.28. 3. During DEAE chromatography of buffer extracts as well as of purified samples of cytochrome c (550; H. stellata ) two bands were formed. Chromatographic experiments performed with pure samples of cytochrome c (550; H. stellata ) and with horse heart cytochrome c from a commercial source suggest that this second band in the DEAE chromatographies is due to protein denaturation during the purification procedure. 4. Cytochrome c (550; H. stellata ) is similar to cytochromes of microorganisms belonging to the families Cryptococcaceae and Moniliaceae and although it is also spectrophotometrically similar to cytochrome c from horse heart it has a highar molecular weight and midpoint redox potential. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Microbiology Springer Journals

Purification and properties of cytochrome c (550; Hemispora stellata )

Archives of Microbiology , Volume 88 (1) – Mar 1, 1973

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References (21)

Publisher
Springer Journals
Copyright
Copyright © 1973 by Springer-Verlag
Subject
Life Sciences; Biotechnology; Biochemistry, general; Cell Biology; Ecology; Microbial Ecology; Microbiology
ISSN
0302-8933
eISSN
1432-072X
DOI
10.1007/BF00408837
Publisher site
See Article on Publisher Site

Abstract

203 88 88 1 1 J. Francisco T. Sala Justino Burgos Facultad de Veterinaria Cátedra de Bioquímica y Tecnología de los Alimentos León Spain Summary 1. A water soluble cytochrome c from Hemispora stellata has been obtained in a highly purified state by a series of steps involving (NH 4 ) 2 SO 4 precipitation and DEAE chromatography. 2. Absorption spectrum of the highly purified cytochrome c (550; H. stellata ) in the reduced state shows maxima at 550, 520, 416, 357–362 nm and 310–320 nm. It has a symmetrical α band and ratios of α red. /prot. ox and Soret red /α red. are 1 and 6.4 respectively. The purified cytochrome has a mesoheme prosthetic group, its molecular weight is about 17500, its isoelectric point is at pH higher than 8.6 and its É o is+0.28. 3. During DEAE chromatography of buffer extracts as well as of purified samples of cytochrome c (550; H. stellata ) two bands were formed. Chromatographic experiments performed with pure samples of cytochrome c (550; H. stellata ) and with horse heart cytochrome c from a commercial source suggest that this second band in the DEAE chromatographies is due to protein denaturation during the purification procedure. 4. Cytochrome c (550; H. stellata ) is similar to cytochromes of microorganisms belonging to the families Cryptococcaceae and Moniliaceae and although it is also spectrophotometrically similar to cytochrome c from horse heart it has a highar molecular weight and midpoint redox potential.

Journal

Archives of MicrobiologySpringer Journals

Published: Mar 1, 1973

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