Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Production of reactive oxygen species and induction of signaling pathways for the ACO gene expressions in tomato plants triggered by the volatile organic compound ether

Production of reactive oxygen species and induction of signaling pathways for the ACO gene... Diethyl ether (ether), a volatile organic compound, is widely used as an industrial solvent and easily released to the environment. Acute exposure of tomato plants to high concentrations of ether caused young leaves to curl. Histochemical analyses revealed that superoxide anion ( • O 2 − ) and hydrogen peroxide were formed sequentially by ether, and that • O 2 − was the major ROS produced in response to ether exposure. We observed cell death by microscopic inspection of Evans blue-stained samples, following fumigation with ether for 6 h. The ethylene biosynthetic gene, 1-aminocyclopropane-1-carboxylic acid oxidase (ACO), was induced as early as 15–30 min after ether fumigation and could be activated at ether concentration as low as 1 μL/L. Induction of ACO gene expression occurred simultaneously with ROS accumulation and coincided with the occurrence of cell death. Simultaneous treatment of tomato plants with mechanical wounding and ether induced differential expression of the ACO gene family. Ether strongly induced ACO4 and moderately induced ACO1, whereas mechanical wounding strongly induced ACO1 and slightly induced ACO4. Induction of the ACO gene family by ether occurred via different signaling pathways. While the ACO1 gene was induced via protein phosphorylation, the ACO4 gene was induced through protein dephosphorylation. Induction of ACO1 and ACO4 might be through MPK1, MPK2, MPK3, and PP2Ac1. These results suggest that the cellular responses of tomato plants to ether are different from the plant responses to ozone, and that tomato plants respond to different air pollutants through different perceptions and downstream signaling pathways. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Cell Reports Springer Journals

Production of reactive oxygen species and induction of signaling pathways for the ACO gene expressions in tomato plants triggered by the volatile organic compound ether

Plant Cell Reports , Volume 30 (4) – Apr 1, 2011

Loading next page...
 
/lp/springer-journals/production-of-reactive-oxygen-species-and-induction-of-signaling-BshmAsm6nG

References (48)

Publisher
Springer Journals
Copyright
Copyright © 2011 by Springer-Verlag
Subject
Life Sciences; Cell Biology; Plant Biochemistry; Plant Sciences ; Biotechnology
ISSN
0721-7714
eISSN
1432-203X
DOI
10.1007/s00299-010-0976-8
pmid
21170714
Publisher site
See Article on Publisher Site

Abstract

Diethyl ether (ether), a volatile organic compound, is widely used as an industrial solvent and easily released to the environment. Acute exposure of tomato plants to high concentrations of ether caused young leaves to curl. Histochemical analyses revealed that superoxide anion ( • O 2 − ) and hydrogen peroxide were formed sequentially by ether, and that • O 2 − was the major ROS produced in response to ether exposure. We observed cell death by microscopic inspection of Evans blue-stained samples, following fumigation with ether for 6 h. The ethylene biosynthetic gene, 1-aminocyclopropane-1-carboxylic acid oxidase (ACO), was induced as early as 15–30 min after ether fumigation and could be activated at ether concentration as low as 1 μL/L. Induction of ACO gene expression occurred simultaneously with ROS accumulation and coincided with the occurrence of cell death. Simultaneous treatment of tomato plants with mechanical wounding and ether induced differential expression of the ACO gene family. Ether strongly induced ACO4 and moderately induced ACO1, whereas mechanical wounding strongly induced ACO1 and slightly induced ACO4. Induction of the ACO gene family by ether occurred via different signaling pathways. While the ACO1 gene was induced via protein phosphorylation, the ACO4 gene was induced through protein dephosphorylation. Induction of ACO1 and ACO4 might be through MPK1, MPK2, MPK3, and PP2Ac1. These results suggest that the cellular responses of tomato plants to ether are different from the plant responses to ozone, and that tomato plants respond to different air pollutants through different perceptions and downstream signaling pathways.

Journal

Plant Cell ReportsSpringer Journals

Published: Apr 1, 2011

There are no references for this article.