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Preparative Procedures and Culture Medium Affect the Success of Cryostorage of Holostemma annulare Shoot Tips

Preparative Procedures and Culture Medium Affect the Success of Cryostorage of Holostemma... Shoot tip cryopreservation of Holostemma annulare, an endangered medicinal plant was carried out using Murashige-Skoog (MS) medium containing mM NH+ 4+NO− 3; 20.6+39.4 (MS-1), 2.6+18.8 (MS-2) or 0.0+18.8 (MS-3). The three media combinations were tested during four preparative procedures viz.: development of cultures; preconditioning of shoot tip cuttings; preculture of encapsulated shoot tips; and post-freeze recovery to understand the most critical phase of NH4NO3 sensitivity. MS-1 used during the initial three preparative steps supported 10.9–16.6% post-freeze recovery of cryopreserved shoot tips. Development of culture in MS-1 and subsequent passages (2nd, 3rd and 4th preparative steps) in MS-2 or MS-3 improved the recovery rate to 26.4–35.8%. MS-3 used throughout the steps favoured 38.5% recovery. Shoot tips from shoot cultures raised in MS-2 upon preconditioning in MS-2 or MS-3 and subsequent preculture of encapsulated shoot tips and post-freeze recovery culture in MS-3 showed maximum regeneration (55%). MS-2 used throughout the procedure supported 48% regeneration of cryopreserved shoot tips. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Cell, Tissue and Organ Culture Springer Journals

Preparative Procedures and Culture Medium Affect the Success of Cryostorage of Holostemma annulare Shoot Tips

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References (16)

Publisher
Springer Journals
Copyright
Copyright © 2004 by Kluwer Academic Publishers
Subject
Life Sciences; Plant Sciences; Plant Physiology
ISSN
0167-6857
eISSN
1573-5044
DOI
10.1023/B:TICU.0000007283.28280.a3
Publisher site
See Article on Publisher Site

Abstract

Shoot tip cryopreservation of Holostemma annulare, an endangered medicinal plant was carried out using Murashige-Skoog (MS) medium containing mM NH+ 4+NO− 3; 20.6+39.4 (MS-1), 2.6+18.8 (MS-2) or 0.0+18.8 (MS-3). The three media combinations were tested during four preparative procedures viz.: development of cultures; preconditioning of shoot tip cuttings; preculture of encapsulated shoot tips; and post-freeze recovery to understand the most critical phase of NH4NO3 sensitivity. MS-1 used during the initial three preparative steps supported 10.9–16.6% post-freeze recovery of cryopreserved shoot tips. Development of culture in MS-1 and subsequent passages (2nd, 3rd and 4th preparative steps) in MS-2 or MS-3 improved the recovery rate to 26.4–35.8%. MS-3 used throughout the steps favoured 38.5% recovery. Shoot tips from shoot cultures raised in MS-2 upon preconditioning in MS-2 or MS-3 and subsequent preculture of encapsulated shoot tips and post-freeze recovery culture in MS-3 showed maximum regeneration (55%). MS-2 used throughout the procedure supported 48% regeneration of cryopreserved shoot tips.

Journal

Plant Cell, Tissue and Organ Culture – Springer Journals

Published: Oct 4, 2004

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