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References (23)
-
Y Kon, H Tsukada, T Hasegawa, K Igarashi, K Wada, E Suzuki, M Arakawa, F Gejyo (1991)
The role of Pseudomonas aeruginosa elastase as a potent inflammatory factor in a rat air pouch inflammation modelFEMS Immunol Med Microbiol, 15
-
Min-Kuei Cheng, S. Takenaka, Shunsuke Aoki, S. Murakami, K. Aoki (2009)
Purification and characterization of an eggshell membrane decomposing protease from Pseudomonas aeruginosa strain ME-4.Journal of bioscience and bioengineering, 107 4
-
Y. Kon, H. Tsukada, T. Hasegawa, K. Igarashi, K. Wada, E. Suzuki, M. Arakawa, F. Gejyo (1999)
The role of Pseudomonas aeruginosa elastase as a potent inflammatory factor in a rat air pouch inflammation model.FEMS immunology and medical microbiology, 25 3
-
(2008)
Hydrolysis chicken egg shell membrane by proteases and production of the hydrolyzed membrane -
P. Braun, J. Tommassen, A. Filloux (1996)
Role of the propeptide in folding and secretion of elastase of Pseudomonas aeruginosaMolecular Microbiology, 19
-
S. Ishikawa, K. Suyama, K. Arihara, M. Itoh (2002)
Uptake and recovery of gold ions from electroplating wastes using eggshell membrane.Bioresource technology, 81 3
-
A. Schmidtchen, E. Holst, H. Tapper, L. Björck (2003)
Elastase-producing Pseudomonas aeruginosa degrade plasma proteins and extracellular products of human skin and fibroblasts, and inhibit fibroblast growth.Microbial pathogenesis, 34 1
-
Kemel Jellouli, A. Bayoudh, L. Manni, Rym Agrebi, M. Nasri (2008)
Purification, biochemical and molecular characterization of a metalloprotease from Pseudomonas aeruginosa MN7 grown on shrimp wastesApplied Microbiology and Biotechnology, 79
-
E. Kessler, M. Safrin, M. Peretz, Y. Burstein (1992)
Identification of cleavage sites involved in proteolytic processing of Pseudomonas aeruginosa preproelastaseFEBS Letters, 299
-
K. Suyama, Yoshitaka Fukazawa, Yoshiyaki Umetsu (1994)
A new biomaterial, hen egg shell membrane, to eliminate heavy metal ion from their dilute waste solutionApplied Biochemistry and Biotechnology, 45-46
-
Ogino, Yokoo, Watanabe, Ishikawa (2000)
Cloning and sequencing of a gene of organic solvent-stable protease secreted from Pseudomonas aeruginosa PST-01 and its expression in Escherichia coli.Biochemical engineering journal, 5 3
-
Robert Bever, B. Iglewski (1988)
Molecular characterization and nucleotide sequence of the Pseudomonas aeruginosa elastase structural geneJournal of Bacteriology, 170
-
M. Rozs, L. Manczinger, C. Vágvölgyi, F. Kevei, A. Hochkoeppler, A. Rodríguez (2001)
Fermentation characteristics and secretion of proteases of a new keratinolytic strain of Bacillus licheniformisBiotechnology Letters, 23
-
P. Schad, R. Bever, T. Nicas, F. Leduc, L. Hanne, B. Iglewski (1987)
Cloning and characterization of elastase genes from Pseudomonas aeruginosaJournal of Bacteriology, 169
-
(2012)
Biotechnol Lett -
Tamao Ino, M. Hattori, Tadashi Yoshida, S. Hattori, K. Yoshimura, Koji Takahashi (2006)
Improved Physical and Biochemical Features of a Collagen Membrane by Conjugating with Soluble Egg Shell Membrane ProteinBioscience, Biotechnology, and Biochemistry, 70
-
Takuo Nakano, NI Ikawa, L. Ozimek (2003)
Chemical composition of chicken eggshell and shell membranes.Poultry science, 82 3
-
K Jellouli, A Bayoudh, L Manni, R Agrebi, M Nasri (2008)
Purification, biochemical and molecular characterization of a metalloprotease from Pseudomonas aeruginosa MN7 grown on shrimp wastesAppl Microbiol Biotechnol, 79
-
Y. Dauphin, J. Cuif, M. Salomé, J. Susini, C. Williams (2006)
Microstructure and chemical composition of giant avian eggshellsAnalytical and Bioanalytical Chemistry, 386
-
Megumi Kuba, Kumi Tanaka, S. Tawata, Y. Takeda, M. Yasuda (2003)
Angiotensin I-Converting Enzyme Inhibitory Peptides Isolated from Tofuyo Fermented Soybean FoodBioscience, Biotechnology, and Biochemistry, 67
-
Richa Sharma, Rani Gupta (2010)
Extracellular expression of keratinase Ker P from Pseudomonas aeruginosa in E. coliBiotechnology Letters, 32
-
A Gupta, S Ray, S Kapoor, SK Khare (2008)
Solvent-stable Pseudomonas aeruginosa PseA protease gene: identification, molecular characterization, phylogenetic and bioinformatic analysis to study reasons for solvent stabilityJ Mol Microbiol Biotechnol, 15
-
Anshu Gupta, S. Ray, S. Kapoor, S. Khare (2007)
Solvent-Stable Pseudomonas aeruginosa PseA Protease Gene: Identification, Molecular Characterization, Phylogenetic and Bioinformatic Analysis to Study Reasons for Solvent StabilityJournal of Molecular Microbiology and Biotechnology, 15
- Publisher
- Springer Journals
- Copyright
- Copyright © 2012 by Springer Science+Business Media B.V.
- Subject
- Life Sciences; Applied Microbiology; Biochemistry, general; Microbiology; Biotechnology
- ISSN
- 0141-5492
- eISSN
- 1573-6776
- DOI
- 10.1007/s10529-012-0861-3
- pmid
- 22286207
- Publisher site
- See Article on Publisher Site
Abstract
Eggshell membrane is a mechanically stable and insoluble cross-linked fibrous protein. Pseudomonas aeruginosa strain ME-4 synthesizes a metalloprotease that degrades the eggshell membrane. We cloned the encoding gene in Escherichia coli. The recombinant protease, over-expressed in E. coli, was inactive but addition of acetone to crude cell extracts restored the activity and removed many E. coli proteins. We purified the active, acetone-treated protease to homogeneity in a single chromatography step with 57% recovery. The recombinant protease partially hydrolyzed eggshell membrane and produced more soluble peptides and proteins than commercial elastase, α-chymotrypsin, and collagenase. The soluble peptides produced from hydrolyzed eggshell membrane inhibited angiotensin-I-converting enzyme activity. The degradation of eggshell membrane by the recombinant elastase could be applied to the production of soluble bioactive peptides.
Journal
Biotechnology Letters – Springer Journals
Published: Jan 29, 2012