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B. Frei, L. England, B. Ames (1989)
Ascorbate is an outstanding antioxidant in human blood plasma.Proceedings of the National Academy of Sciences of the United States of America, 86 16
D. Steinberg, S. Parthasarathy, T. Carew, J. Khoo, J. Witztum (1989)
Beyond cholesterol. Modifications of low-density lipoprotein that increase its atherogenicity.The New England journal of medicine, 320 14
W. Jones, Xia Li, Z. Qu, L. Perriott, R. Whitesell, J. May (2002)
Uptake, recycling, and antioxidant actions of α-lipoic acid in endothelial cellsFree Radical Biology and Medicine, 33
U. Steinbrecher, S. Parthasarathy, D. Leake, J. Witztum, D. Steinberg (1984)
Modification of low density lipoprotein by endothelial cells involves lipid peroxidation and degradation of low density lipoprotein phospholipids.Proceedings of the National Academy of Sciences of the United States of America, 81 12
P. Hissin, R. Hilf (1976)
A fluorometric method for determination of oxidized and reduced glutathione in tissues.Analytical biochemistry, 74 1
B. Winkler, S. Orselli, T. Rex (1994)
The redox couple between glutathione and ascorbic acid: a chemical and physiological perspective.Free radical biology & medicine, 17 4
J. Steinberg (1989)
Book ReviewAmbulatory Pediatric CareThe New England Journal of Medicine, 320
L. Packer, E. Witt, H. Tritschler (1995)
alpha-Lipoic acid as a biological antioxidant.Free radical biology & medicine, 19 2
C. Edgell, C. McDonald, J. Graham (1983)
Permanent cell line expressing human factor VIII-related antigen established by hybridization.Proceedings of the National Academy of Sciences of the United States of America, 80 12
L Packer, EH Witt, HJ Tritschler (1995)
α-Lipoic acid as a biological antioxidantFree Radic Biol Med, 19
M. Pech-Amsellem, I. Myara, I. Pico, C. Maziére, J. Mazière, N. Moatti (1996)
Oxidative modifications of low-density lipoproteins (LDL) by the human endothelial cell line EA.hy 926Experientia, 52
Sashwati Roy, L. Packer (1998)
Redox regulation of cell functions by α‐lipoate: biochemical and molecular aspectsBioFactors, 7
C. Michiels (2003)
Endothelial cell functionsJournal of Cellular Physiology, 196
P. Jocelyn (1967)
The standard redox potential of cysteine-cystine from the thiol-disulphide exchange reaction with glutathione and lipoic acid.European journal of biochemistry, 2 3
J. May, Z. Qu (2005)
Transport and intracellular accumulation of vitamin C in endothelial cells: relevance to collagen synthesis.Archives of biochemistry and biophysics, 434 1
A. Nègre-Salvayre, L. Mabile, J. Delchambre, R. Salvayre (2007)
α-tocopherol, ascorbic acid, and rutin inhibit synergistically the copper-promoted LDL oxidation and the cytotoxicity of oxidized LDL to cultured endothelial cellsBiological Trace Element Research, 47
W. Wells, Dianpeng Xu (1994)
Dehydroascorbate reductionJournal of Bioenergetics and Biomembranes, 26
D. Han, G. Handelman, L. Marcocci, C. Sen, S. Roy, H. Kobuchi, H. Tritschler, L. Flohé, L. Packer (1997)
Lipoic acid increases de novo synthesis of cellular glutathione by improving cystine utilizationBioFactors, 6
J. Teichert, R. Hermann, P. Ruus, R. Preiss (2003)
Plasma Kinetics, Metabolism, and Urinary Excretion of Alpha‐Lipoic Acid following Oral Administration in Healthy VolunteersThe Journal of Clinical Pharmacology, 43
L. Packer, H. Tritschler (1996)
Alpha-lipoic acid: the metabolic antioxidant.Free radical biology & medicine, 20 4
R. Siow, H. Sato, D. Leake, T. Ishii, S. Bannai, G. Mann (1999)
Induction of antioxidant stress proteins in vascular endothelial and smooth muscle cells: protective action of vitamin C against atherogenic lipoproteins.Free radical research, 31 4
K. Retsky, Ken Chen, J. Zeind, B. Frei, B. Frei (1999)
Inhibition of copper-induced LDL oxidation by vitamin C is associated with decreased copper-binding to LDL and 2-oxo-histidine formation.Free radical biology & medicine, 26 1-2
W. Sattler, D. Mohr, R. Stocker (1994)
Rapid isolation of lipoproteins and assessment of their peroxidation by high-performance liquid chromatography postcolumn chemiluminescence.Methods in enzymology, 233
Williams Kj, I. Tabas (1999)
Atherosclerosis--an inflammatory disease.The New England journal of medicine, 340 24
B. Tebbe, Shuling Wu, C. Geilen, Jürgen Eberle, V. Kodelja, C. Orfanos (1997)
L-ascorbic acid inhibits UVA-induced lipid peroxidation and secretion of IL-1alpha and IL-6 in cultured human keratinocytes in vitro.The Journal of investigative dermatology, 108 3
V. Kagan, E. Serbinova, T. Forte, G. Scita, L. Packer (1992)
Recycling of vitamin E in human low density lipoproteins.Journal of lipid research, 33 3
J. Bauer, M. Margolis, C. Schreiner, C. Edgell, J. Azizkhan, E. Lazarowski, R. Juliano (1992)
In vitro model of angiogenesis using a human endothelium‐derived permanent cell line: Contributions of induced gene expression, G‐proteins, and integrinsJournal of Cellular Physiology, 153
J. May, Z. Qu, Xia Li (2003)
Ascorbic acid blunts oxidant stress due to menadione in endothelial cells.Archives of biochemistry and biophysics, 411 1
E. Niki, N. Noguchi, Hidevasu Tsuchihashi, N. Gotoh (1995)
Interaction among vitamin C, vitamin E, and beta-carotene.The American journal of clinical nutrition, 62 6 Suppl
Hjordis Thort, Martyn Smith, Pia Hartzells, Giorgio L, Sarah Jewelll, Sten Orreniusl, Italy (1982)
The metabolism of menadione (2-methyl-1,4-naphthoquinone) by isolated hepatocytes. A study of the implications of oxidative stress in intact cells.The Journal of biological chemistry, 257 20
D Steinberg, S Parthasarathy, TE Carew (1989)
Modifications of low-density lipoprotein that increase its atherogenicityN Engl J Med, 320
S. Mendiratta, Z. Qu, J. May (1998)
Erythrocyte ascorbate recycling: antioxidant effects in blood.Free radical biology & medicine, 24 5
Antonio Martín, B. Frei (1997)
Both intracellular and extracellular vitamin C inhibit atherogenic modification of LDL by human vascular endothelial cells.Arteriosclerosis, thrombosis, and vascular biology, 17 8
G. Totzke, C. Metzner, G. Ulrich-Merzenich, Y. Ko, A. Sachinidis, H. Vetter (2001)
Effect of vitamin E and vitamin C on the DNA synthesis of human umbilical arterial endothelial cellsEuropean Journal of Nutrition, 40
S. Matsugo, T. Konishi, D. Matsuo, H. Tritschler, L. Packer (1996)
Reevaluation of superoxide scavenging activity of dihydrolipoic acid and its analogues by chemiluminescent method using 2-methyl-6-[p-methoxyphenyl]-3,7-dihydroimidazo-[1,2-a]pyrazine-3-one (MCLA) as a superoxide probe.Biochemical and biophysical research communications, 227 1
J. May, Z. Qu, S. Mendiratta (1998)
Protection and Recycling of α-Tocopherol in Human Erythrocytes by Intracellular Ascorbic AcidArchives of Biochemistry and Biophysics, 349
Both α-lipoic acid (LA) and ascorbic acid (vitamin C) have been shown to improve endothelial dysfunction, a precursor of atherosclerosis. Since oxidant stress can cause endothelial dysfunction, we tested the interaction and efficacy of these antioxidants in preventing oxidant damage to lipids due to both intra- and extracellular oxidant stresses in EA.hy926 endothelial cells. LA spared intracellular ascorbate in culture and in response to an intracellular oxidant stress induced by the redox cycling agent menadione. Extracellular oxidant stress generated by incubating cells for 2 h in with 0.2 mg/ml LDL and 5 μM Cu2+ caused a time-dependent increase of the lipid peroxidation product malondialdehyde in both cells and LDL, preceded by rapid disappearance of` α-tocopherol in LDL. α-Lipoic acid at concentrations of 40–80 μM blunted these effects. Similarly, intracellular ascorbate concentrations of 1–2 mM also prevented Cu2+-induced lipid peroxidation in LDL and cells. Cu2+-dependent oxidation of LDL in the presence of ascorbate-loaded cells decreased intracellular ascorbate by 20%, but this decrease was not reversed by LA. Both LA and ascorbate protect endothelial cells and LDL from either intra- or extracellular oxidant stress, but that LA does not spare ascorbate in oxidatively stressed cells.
Molecular and Cellular Biochemistry – Springer Journals
Published: Nov 16, 2007
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