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Isolation and characterization of potato-tomato somatic hybrids using an amylose-free potato mutant as parental genotype

Isolation and characterization of potato-tomato somatic hybrids using an amylose-free potato... 122 85 85 2 3 E. Jacobsen P. Reinhout J. E. M. Bergervoet J. de Looff P. E. Abidin D. J. Huigen M. S. Ramanna Department of Plant Breeding Agricultural University NL-6700 AJ Wageningen The Netherlands Summary Using different genotypes of tomato and diploid potato, possessing alien selectable markers as well as endogenous markers, very high frequencies of protoplast fusion hybrids were obtained. One endogenous genetic marker, the amylose-free ( amf ) mutant of potato, was helpful not only for the confirmation of fusion products but also for the study of genetic complementation and the segregation of amylose-free starch in microspores. Cytological analysis of the fusion hybrids indicated that except for one which was hexaploid, all of them had a perfectly balanced chromosome number of allotetraploid constitution (2n = 4x = 48). Despite normal chromosome pairing and a diploid behaviour, the microspores in some of the fusion hybrids segregated for the recessive amf -locus. This anomalous segregation of a recessive character in these hybrids was shown not to be due to chromosome elimination or to the absence of the wild-type tomato Amf gene. Although all fusion hybrids were totally sterile, the hexaploid produced stainable pollen and berries with badly developed seeds. Embryo rescue has so far failed to produce backcross progeny. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png TAG Theoretical and Applied Genetics Springer Journals

Isolation and characterization of potato-tomato somatic hybrids using an amylose-free potato mutant as parental genotype

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References (29)

Publisher
Springer Journals
Copyright
Copyright © 1992 by Springer-Verlag
Subject
Life Sciences; Biotechnology; Agriculture; Biochemistry, general; Plant Biochemistry; Plant Sciences; Plant Genetics & Genomics
ISSN
0040-5752
eISSN
1432-2242
DOI
10.1007/BF00222854
pmid
24197299
Publisher site
See Article on Publisher Site

Abstract

122 85 85 2 3 E. Jacobsen P. Reinhout J. E. M. Bergervoet J. de Looff P. E. Abidin D. J. Huigen M. S. Ramanna Department of Plant Breeding Agricultural University NL-6700 AJ Wageningen The Netherlands Summary Using different genotypes of tomato and diploid potato, possessing alien selectable markers as well as endogenous markers, very high frequencies of protoplast fusion hybrids were obtained. One endogenous genetic marker, the amylose-free ( amf ) mutant of potato, was helpful not only for the confirmation of fusion products but also for the study of genetic complementation and the segregation of amylose-free starch in microspores. Cytological analysis of the fusion hybrids indicated that except for one which was hexaploid, all of them had a perfectly balanced chromosome number of allotetraploid constitution (2n = 4x = 48). Despite normal chromosome pairing and a diploid behaviour, the microspores in some of the fusion hybrids segregated for the recessive amf -locus. This anomalous segregation of a recessive character in these hybrids was shown not to be due to chromosome elimination or to the absence of the wild-type tomato Amf gene. Although all fusion hybrids were totally sterile, the hexaploid produced stainable pollen and berries with badly developed seeds. Embryo rescue has so far failed to produce backcross progeny.

Journal

TAG Theoretical and Applied Genetics – Springer Journals

Published: Nov 1, 1992

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