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IMS Method Performance Analyses for Giardia in Water Under Differing Conditions

IMS Method Performance Analyses for Giardia in Water Under Differing Conditions Immunomagnetic separation (IMS) has been specified as a standard method for the measurement of Giardia under USEPA Method 1623. In this study, IMS was evaluated on the basis of recovery efficiencies for Giardia cysts under various IMS operation conditions. Significant change in recovery was observed by altering the debris ratio of water samples. Notably, cyst recovery efficiencies utilizing IMS dropped with increased turbidity, and results for varying dosages of magnetic beads and cysts indicate that 1 / 100 immunomagnetic beads is sufficient to conjugate large numbers of cysts. Changing vessel volume and replacing the sample buffer had no significant effect on cyst recovery efficiencies. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Environmental Monitoring and Assessment Springer Journals

IMS Method Performance Analyses for Giardia in Water Under Differing Conditions

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References (16)

Publisher
Springer Journals
Copyright
Copyright © 2006 by Springer Science+Business Media B.V.
Subject
Environment; Monitoring/Environmental Analysis; Environmental Management; Ecotoxicology; Atmospheric Protection/Air Quality Control/Air Pollution; Ecology
ISSN
0167-6369
eISSN
1573-2959
DOI
10.1007/s10661-006-9462-8
pmid
17106771
Publisher site
See Article on Publisher Site

Abstract

Immunomagnetic separation (IMS) has been specified as a standard method for the measurement of Giardia under USEPA Method 1623. In this study, IMS was evaluated on the basis of recovery efficiencies for Giardia cysts under various IMS operation conditions. Significant change in recovery was observed by altering the debris ratio of water samples. Notably, cyst recovery efficiencies utilizing IMS dropped with increased turbidity, and results for varying dosages of magnetic beads and cysts indicate that 1 / 100 immunomagnetic beads is sufficient to conjugate large numbers of cysts. Changing vessel volume and replacing the sample buffer had no significant effect on cyst recovery efficiencies.

Journal

Environmental Monitoring and AssessmentSpringer Journals

Published: Nov 15, 2006

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