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- Publisher
- Springer Journals
- Copyright
- Copyright © 1997 by Kluwer Academic Publishers
- Subject
- Life Sciences; Plant Sciences; Plant Physiology
- ISSN
- 0167-6857
- eISSN
- 1573-5044
- DOI
- 10.1023/A:1005962913491
- Publisher site
- See Article on Publisher Site
Abstract
Agrobacterium rhizogenes A4 harboring the agropine type root-inducing plasmid (pRiA4) induced hairy roots on stem sections of gentian plants. Transgenic gential plants were regenerated from the hairy roots at a frequency of 19% adventitious shoot regeneration on Murashige and Skoog medium containing 30 g l-1 sucrose, 10 mg l-1 N-phenyl-N′-1,2,3-thiadiazol-5-yl urea, 1 mg l-1 1-naphthaleneacetic acid and 2 g l-1 gellan gum. The shoots were grown to maturity in a growth chamber after acclimatization. The mature transformed plants showed distinct variations in their phenotypes, such as dwarfness. Polymerase chain reaction and Southern blot analysis revealed stable integration of transferred DNA.
Journal
Plant Cell, Tissue and Organ Culture – Springer Journals
Published: Sep 29, 2004