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Factors affecting the induction of pollen plants of intergeneric hybrids of Triticum aestivum X Triticum-Agropyron

Factors affecting the induction of pollen plants of intergeneric hybrids of Triticum aestivum X... 122 70 70 3 3 Zhuang Jiajun Jia Xu Chen Guoqing Sun Shancheng Institute of Genetics Academia Sinica Beijing China Institute of Corn, Guangxi Zhuang Autonomous Regin Nanning China Summary Experimental results showed that the use of potato extract as a basic component of culture medium had a promoting effect on producing calli in anther culture of the intergeneric hybrids of Triticum aestivum × Triticum-Agropyron (intermediate type). The induction frequencies of pollen callus on the Potato-II medium containing potato extract as the main component was much higher than that found on N 6 and W 5 media. The induction frequencies of pollen callus and green plantlets in four intergeneric hybrid material inoculated at the late-uninucleate pollen stage were all higher than those inoculated at the mid-uninucleate stage. Appropriate increases in culture temperature significantly increased pollen callus induction frequencies of the intergeneric hybrids. The genotype and physiological state of anther donor plants also influenced pollen callus and green plantlet induction frequencies. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png TAG Theoretical and Applied Genetics Springer Journals

Factors affecting the induction of pollen plants of intergeneric hybrids of Triticum aestivum X Triticum-Agropyron

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References (11)

Publisher
Springer Journals
Copyright
Copyright © 1985 by Springer-Verlag
Subject
Life Sciences; Biotechnology; Agriculture; Biochemistry, general; Plant Biochemistry; Plant Sciences; Plant Genetics & Genomics
ISSN
0040-5752
eISSN
1432-2242
DOI
10.1007/BF00304914
pmid
24252924
Publisher site
See Article on Publisher Site

Abstract

122 70 70 3 3 Zhuang Jiajun Jia Xu Chen Guoqing Sun Shancheng Institute of Genetics Academia Sinica Beijing China Institute of Corn, Guangxi Zhuang Autonomous Regin Nanning China Summary Experimental results showed that the use of potato extract as a basic component of culture medium had a promoting effect on producing calli in anther culture of the intergeneric hybrids of Triticum aestivum × Triticum-Agropyron (intermediate type). The induction frequencies of pollen callus on the Potato-II medium containing potato extract as the main component was much higher than that found on N 6 and W 5 media. The induction frequencies of pollen callus and green plantlets in four intergeneric hybrid material inoculated at the late-uninucleate pollen stage were all higher than those inoculated at the mid-uninucleate stage. Appropriate increases in culture temperature significantly increased pollen callus induction frequencies of the intergeneric hybrids. The genotype and physiological state of anther donor plants also influenced pollen callus and green plantlet induction frequencies.

Journal

TAG Theoretical and Applied GeneticsSpringer Journals

Published: Jun 1, 1985

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