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Expression patterns of WRKY genes in di-haploid Populus simonii × P . nigra in response to salinity stress revealed by quantitative real-time PCR and RNA sequencing

Expression patterns of WRKY genes in di-haploid Populus simonii × P . nigra in response to... To understand the expression patterns of poplar WRKY genes under salinity stress, 51 of the 119 WRKY genes were selected from di-haploid Populus simonii × P . nigra by quantitative real-time PCR (qRT-PCR). We used qRT-PCR to profile the expression of the top 13 genes under salinity stress across seven time points, and employed RNA-Seq platforms to cross-validate it. Results demonstrated that all the 13 WRKY genes were expressed in root, stem, and leaf tissues, but their expression levels and overall patterns varied notably in these tissues. Regarding overall gene expression in roots, the 13 genes were significantly highly expressed at all six time points after the treatment, reaching the plateau of expression at hour 9. In leaves, the 13 genes were similarly up-regulated from 3 to 12 h in response to NaCl treatment. In stems, however, expression levels of the 13 genes did not show significant changes after the NaCl treatment. Regarding individual gene expression across the time points and the three tissues, the 13 genes can be classified into three clusters: the lowly expressed Cluster 1 containing PthWRKY28, 45 and 105 ; intermediately expressed Clusters 2 including PthWRKY56 , 88 and 116 ; and highly expressed Cluster 3 consisting of PthWRKY41, 44, 51, 61, 62, 75 and 106 . In general, genes in Cluster 2 and 3 displayed a dynamic pattern of “induced amplification—recovering”, suggesting that these WRKY genes and corresponding pathways may play a critical role in mediating salt response and tolerance in a dynamic and tissue-specific manner. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Cell Reports Springer Journals

Expression patterns of WRKY genes in di-haploid Populus simonii × P . nigra in response to salinity stress revealed by quantitative real-time PCR and RNA sequencing

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References (46)

Publisher
Springer Journals
Copyright
Copyright © 2014 by Springer-Verlag Berlin Heidelberg
Subject
Life Sciences; Cell Biology; Plant Sciences; Biotechnology; Plant Biochemistry
ISSN
0721-7714
eISSN
1432-203X
DOI
10.1007/s00299-014-1647-y
pmid
24969399
Publisher site
See Article on Publisher Site

Abstract

To understand the expression patterns of poplar WRKY genes under salinity stress, 51 of the 119 WRKY genes were selected from di-haploid Populus simonii × P . nigra by quantitative real-time PCR (qRT-PCR). We used qRT-PCR to profile the expression of the top 13 genes under salinity stress across seven time points, and employed RNA-Seq platforms to cross-validate it. Results demonstrated that all the 13 WRKY genes were expressed in root, stem, and leaf tissues, but their expression levels and overall patterns varied notably in these tissues. Regarding overall gene expression in roots, the 13 genes were significantly highly expressed at all six time points after the treatment, reaching the plateau of expression at hour 9. In leaves, the 13 genes were similarly up-regulated from 3 to 12 h in response to NaCl treatment. In stems, however, expression levels of the 13 genes did not show significant changes after the NaCl treatment. Regarding individual gene expression across the time points and the three tissues, the 13 genes can be classified into three clusters: the lowly expressed Cluster 1 containing PthWRKY28, 45 and 105 ; intermediately expressed Clusters 2 including PthWRKY56 , 88 and 116 ; and highly expressed Cluster 3 consisting of PthWRKY41, 44, 51, 61, 62, 75 and 106 . In general, genes in Cluster 2 and 3 displayed a dynamic pattern of “induced amplification—recovering”, suggesting that these WRKY genes and corresponding pathways may play a critical role in mediating salt response and tolerance in a dynamic and tissue-specific manner.

Journal

Plant Cell ReportsSpringer Journals

Published: Oct 1, 2014

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