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T. Ng, A. Ben-Bassat, J. Zeikus (1981)
Ethanol Production by Thermophilic Bacteria: Fermentation of Cellulosic Substrates by Cocultures of Clostridium thermocellum and Clostridium thermohydrosulfuricumApplied and Environmental Microbiology, 41
R. Lamed, J. Zeikus (1980)
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253 22 22 4 4 Olga Volfová Olga Suchardová Jan Panoš Vladimír Krumphanzl Institute of Microbiology Czechoslovak Academy of Sciences 14220 Prague 4 Czechoslovakia Summary A wild coculture of obligately thermophilic bacteria, including only a single cellulolytic species Clostridium , ferments 2% crystalline cellulose and produces 4.6–5.1 g·l −1 of ethanol at 55°–60° C; that is, 0.96–1.1 moles of ethanol from 1 mole of glucose equivalent of cellulose degraded. However, the ethanol yield decreases with increasing cellulose concentration. Ethanolacetic acid ratio varies around 1 and cannot be influenced by substrate concentration. However, this ratio can be influenced by changing pH and temperature. For the ethanol production from cellulose, neutral and weekly alkaline media with a pH of 7.0–8.0 and a temperature of 55° C are optimal. Experiments in which the coculture was subjected to high ethanol concentrations showed that higher concentrations of added ethanol (up to 20 g·l −1 ) suppress cellulose degradation by 50% and inhibit the actual production of ethanol.
Applied Microbiology and Biotechnology – Springer Journals
Published: Aug 1, 1985
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