Access the full text.
Sign up today, get DeepDyve free for 14 days.
T. Ikeda, D. Kobayashi, F. Matsushita, T. Sagara, K. Niki (1993)
Bioelectrocatalysis at electrodes coated with alcohol dehydrogenase, a quinohemoprotein with heme c serving as a built-in mediator.Journal of Electroanalytical Chemistry, 361
B. Gregg, A. Heller (1991)
Redox polymer films containing enzymes. 1. A redox-conducting epoxy cement: synthesis, characterization, and electrocatalytic oxidation of hydroquinoneThe Journal of Physical Chemistry, 95
C. Bourdillon, R. Lortie, J. Laval (1988)
Gluconic acid production by an mobilized glucose oxidase reactor with electrochemical regeneration of an artificial electron acceptorBiotechnology and Bioengineering, 31
B. Groen, M. Kleef, J. Duine (1986)
Quinohaemoprotein alcohol dehydrogenase apoenzyme from Pseudomonas testosteroni.The Biochemical journal, 234 3
Y. Degani, A. Heller (1989)
Electrical communication between redox centers of glucose oxidase and electrodes via electrostatically and covalently bound redox polymersJournal of the American Chemical Society, 111
E. Stigter, J. Lugt, W. Somers (1997)
Enantioselective oxidation of secondary alcohols by quinohaemoprotein alcohol dehydrogenase from Comamonas testosteroniJournal of Molecular Catalysis B-enzymatic, 2
H. Toyama, A. Fujii, K. Matsushita, E. Shinagawa, M. Ameyama, O. Adachi (1995)
Three distinct quinoprotein alcohol dehydrogenases are expressed when Pseudomonas putida is grown on different alcoholsJournal of Bacteriology, 177
Alfred Haimerl, A. Merz (1986)
Ferrocen-modifizierte Polypyrrolfilme durch elektrochemische Copolymerisation†Angewandte Chemie, 98
V. Pham, R. Phillips (1990)
Effects of substrate structure and temperature on the stereospecificity of secondary alcohol dehydrogenase from Thermoanaerobacter ethanolicusJournal of the American Chemical Society, 112
Y. Kitagawa, K. Kitabatake (1991)
Amperometric detection of alcohol in beer using a flow cell and immobilized alcohol dehydrogenase.Analytical chemistry, 63 20
E. Keinan, E. Hafeli, K. Seth, R. Lamed (1986)
Thermostable enzymes in organic synthesis. 2. Asymmetric reduction of ketones with alcohol dehydrogenase from Thermoanaerobium brockiiJournal of the American Chemical Society, 108
F. Welsh, W. Murray, Ross Williams (1989)
Microbiological and Enzymatic Production of Flavor and Fragrance ChemicalsCritical Reviews in Biotechnology, 9
A. Geerlof, J. Tol, J. Jongejan, J. Duine (1994)
Enantioselective Conversions of the Racemic C3-Alcohol Synthons, Glycidol (2, 3-Epoxy-1-propanol), and Solketal (2, 2-Dimethyl-4-(hydroxymethyl)-1, 3-dioxolane) by Quinohaemoprotein Alcohol Dehydrogenases and Bacteria Containing Such EnzymesBioscience, Biotechnology, and Biochemistry, 58
Shishan. Zhao, R. Lennox (1991)
Pyrroloquinolinequinone enzyme electrode based on the coupling of methanol dehydrogenase to a tetrathiafulvalene-tetracyanoquinodimethane electrodeAnalytical Chemistry, 63
E. Stigter, G. Jong, J. Jongejan, Hans Duine, J. Lugt, W. Somers (1997)
Electron Transfer and Stability of a Quinohaemoprotein Alcohol Dehydrogenase ElectrodeJournal of Chemical Technology & Biotechnology, 68
W. Schuhmann, Heidi Wohlschläger, R. Lammert, H. Schmidt, U. Löffler, H. Wiemhöfer, W. Göpel (1990)
Leaching of dimethylferrocene, a redox mediator in amperometric enzyme electrodesSensors and Actuators B-chemical, 1
A. Geerlof, J. Rakels, A. Straathof, J. Heijnen, J. Jongejan, J. Duine (1994)
Description of the kinetic mechanism and the enantioselectivity of quinohaemoprotein ethanol dehydrogenase from Comamonas testosteroni in the oxidation of alcohols and aldehydes.European journal of biochemistry, 226 2
F. Petry (1997)
Principles and Applications
L. Gorton, E. Csöregi, E. Domínguez, J. Emnéus, G. Jönsson-Pettersson, G. Marko‐Varga, B. Persson (1991)
Selective detection in flow analysis based on the combination of immobilized enzymes and chemically modified electrodesAnalytica Chimica Acta, 250
J. Duine (1991)
Quinoproteins: enzymes containing the quinonoid cofactor pyrroloquinoline quinone, topaquinone or tryptophan-tryptophan quinone.European journal of biochemistry, 200 2
V. Davidson (1993)
Principles and Applications of Quinoproteins
G. Jong, A. Geerlof, J. Stoorvogel, J. Jongejan, S. Vries, J. Duine (1995)
Quinohaemoprotein Ethanol Dehydrogenase from Comamonas testosteroniFEBS Journal, 230
P. Hale, T. Inagaki, H. Karan, Y. Okamoto, T. Skotheim (1989)
A new class of amperometric biosensor incorporating a polymeric electron-transfer mediatorJournal of the American Chemical Society, 111
E. Stigter, G. Jong, J. Jongejan, J. Duine, J. Lugt, W. Somers (1996)
Electron transfer between a quinohemoprotein alcohol dehydrogenase and an electrode via a redox polymer networkEnzyme and Microbial Technology, 18
G. Jong, A. Geerlof, J. Stoorvogel, J. Jongejan, S. Vries, J. Duine (1995)
Quinohaemoprotein ethanol dehydrogenase from Comamonas testosteroni. Purification, characterization, and reconstitution of the apoenzyme with pyrroloquinoline quinone analogues.European journal of biochemistry, 230 3
Quinohaemoprotein alcohol dehydrogenase fromComamonas testosteroni was co-immobilized with a redox polymer (a poly(vinylpyridine) complex functionalized with osmium bis(bipyridine) chloride) on an electrode. The enzyme electrode readily oxidizes primary alcohols and secondary alcohols with maximum current densities varying between 0.43 and 0.98 A m-2 depending on the substrate and the operation temperature. The affinity of the enzyme for aliphatic alcohols increases with the chain length of the substrate (i.e., 1-pentano1 [Km = 0.006 mM] is a much better substrate than ethanol [Km= 2.2 mM]). The same property is observed for secondary alcohols in the series 2-propanol (Km = 22 mM) to 2-octano1 (Km = 0.05 mM). The enzyme electrode is enantioselective in the oxidation of secondary alcohols. A strong preference is observed for the S-2-alcohols; the enantioselectivity increases with increasing chain length. The enantiomeric ratio (E) increases from 13 for (R,S)-2-butanol to approximately 80 for (R,S)-2-heptanol and (R,S)-2-octanol. This makes the enzyme electrode, potentially, a powerful tool for the preparation of a large range of alkanones and/or for the (kinetic) resolution of racemic alcohols.
Applied Biochemistry and Biotechnology – Springer Journals
Published: Dec 14, 2007
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.