Purpose . The goal of this study was to determine the effects of exposure to an HAV peptide (Ac-SHAVSS-NH 2 ) on the protein and gene expression in Caco-2 cells, a model for the intestinal mucosa. Methods . Caco-2 cells were incubated with either 100 or 500 μM of the hexapeptide then evaluated over a 48-h time period. Results . Cell detachment from the monolayer was seen only after 48 h of exposure to the peptide, with the greatest effects occurring with a peptide concentration of 500 μM. Total protein expression of E-cadherin showed a decrease of nearly 20% at the 24-h time point for each concentration examined, whereas no significant changes were detected at the other time points studied. Short term exposure to a 500 μM solution of Ac-SHAVSS-NH 2 caused few changes in gene expression as determined by Affymetrix GeneChip⌖ microarrays; however, longer exposure periods produced numerous changes in the treated cells. The variations in mRNA expression indicate that this HAV peptide has an effect in the E-cadherin signaling pathways. The greatest increases in mRNA expression were found in genes regulating excretion or degradation of the peptide. Conclusions . This work suggests that this HAV peptide produces effects that reach beyond modulation of adhesion.
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Effects of An E-cadherin-Derived Peptide on the Gene Expression of Caco-2 Cells
Anna, Calcagno; Jennifer, Fostel; Eric, Reyner; Ernawati, Sinaga; James, Alston; William, Mattes; Teruna, Siahaan; Joseph, Ware
Follow Pharmaceutical Research
, Volume 21 (11)
Springer Journals – Nov 1, 2004
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