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Effect of ionizing radiation on haemoglobin of marine lamellibranchs

Effect of ionizing radiation on haemoglobin of marine lamellibranchs 227 10 10 3 3 S. Patel B. Patel Health Physics Division Bhabha Atomic Research Centre Bombay India Abstract The effect of ionizing radiation on the iron-linked protein (haemoglobin) of the marine lamellibranchs Anadara granosa ( Linn. ) and Cardita antiquata ( Lam. ) from Bombay waters, India is discussed. Purified haemoglobin solutions were exposed to a 60 Cobalt source delivering a dose of about 4,600 rad/min, at the sample irradiation point. Radiation damage or degradation was measured spectrophometrically by studying changes in the absorption spectra following irradiation in the presence and absence of oxygen. Exposure to ionizing radiation in general caused a decrease in absorption of both haemoglobins, irrespective of location, viz extra-versus intracellular, at Soret (412 mμ), β (540 to 42 mμ) and α (574 to 76 mμ) peaks, and an increase in absorption at 510 and 630 mμ. Upon exposure to a higher dose, O 2 Hb of C. antiquata showed an increase in absorption at the protein peak (280 mμ); exposure to a lower dose, however, resulted in decreased absorption. Furthermore, the changes following irradiation were dependent upon the initial state of the pigment. Oxyhaemoglobin, when exposed to radiation, oxidized to hemiglobin, and hemiglobin reduced to oxyhaemoglobin. The extracellular haemoglobin of high molecular weight (3x10 6 ) of the false cockle C. antiquata was found to be extremely radio-resistant, whereas intracellular haemoglobin of low molecular weight (74,000) of the arcid clam A. granosa was highly radiosensitive, since it could not be exposed to doses exceeding 18,000 r. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Marine Biology Springer Journals

Effect of ionizing radiation on haemoglobin of marine lamellibranchs

Patel, S.; Patel, B.
Marine Biology , Volume 10 (3) – Aug 1, 1971
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References (14)

Publisher
Springer Journals
Copyright
Copyright © 1971 by Springer-Verlag
Subject
Life Sciences; Biomedicine general; Oceanography; Ecology; Microbiology; Zoology
ISSN
0025-3162
eISSN
1432-1793
DOI
10.1007/BF00352818
Publisher site
See Article on Publisher Site

Abstract

227 10 10 3 3 S. Patel B. Patel Health Physics Division Bhabha Atomic Research Centre Bombay India Abstract The effect of ionizing radiation on the iron-linked protein (haemoglobin) of the marine lamellibranchs Anadara granosa ( Linn. ) and Cardita antiquata ( Lam. ) from Bombay waters, India is discussed. Purified haemoglobin solutions were exposed to a 60 Cobalt source delivering a dose of about 4,600 rad/min, at the sample irradiation point. Radiation damage or degradation was measured spectrophometrically by studying changes in the absorption spectra following irradiation in the presence and absence of oxygen. Exposure to ionizing radiation in general caused a decrease in absorption of both haemoglobins, irrespective of location, viz extra-versus intracellular, at Soret (412 mμ), β (540 to 42 mμ) and α (574 to 76 mμ) peaks, and an increase in absorption at 510 and 630 mμ. Upon exposure to a higher dose, O 2 Hb of C. antiquata showed an increase in absorption at the protein peak (280 mμ); exposure to a lower dose, however, resulted in decreased absorption. Furthermore, the changes following irradiation were dependent upon the initial state of the pigment. Oxyhaemoglobin, when exposed to radiation, oxidized to hemiglobin, and hemiglobin reduced to oxyhaemoglobin. The extracellular haemoglobin of high molecular weight (3x10 6 ) of the false cockle C. antiquata was found to be extremely radio-resistant, whereas intracellular haemoglobin of low molecular weight (74,000) of the arcid clam A. granosa was highly radiosensitive, since it could not be exposed to doses exceeding 18,000 r.

Journal

Marine Biology – Springer Journals

Published: Aug 1, 1971

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