Access the full text.
Sign up today, get DeepDyve free for 14 days.
Andreas Zophel, Michael Kennedy, Helmut Beinert, Peter Kroneck (1988)
Investigations on microbial sulfur respirationArchives of Microbiology, 150
T. Brittain, C. Greenwood (1975)
A comparative study of some kinetic and spectral properties of guanidinated and native cytochrome c.The Biochemical journal, 147 1
Shin-ichi Kajie, Y. Anraku (1986)
Purification of a hexaheme cytochrome c552 from Escherichia coli K 12 and its properties as a nitrite reductase.European journal of biochemistry, 154 2
(1975)
Preparation of metalloporhyrins from porphyrins and isolation from natural sources. In: Smith KM (ed) Porphyrins and metalloporphyrisn
(1988)
Ecology of denitrification
P. Janick, L. Siegel (1983)
Electron paramagnetic resonance and optical evidence for interaction between siroheme and the tetranuclear iron-sulfur center (Fe4S4) prosthetic groups in complexes of Escherichia coli sulfite reductase hemoprotein with added ligandsBiochemistry, 22
(1975)
Preparation of metalloporhyrins from porphyrins and isolation from natural sources
A. Zöphel, M. Kennedy, H. Beinert, P. Kroneck (1991)
Investigations on microbial sulfur respiration. Isolation, purification, and characterization of cellular components from Spirillum 5175.European journal of biochemistry, 195 3
J. Heukeshoven, R. Dernick (1985)
Simplified method for silver staining of proteins in polyacrylamide gels and the mechanism of silver stainingELECTROPHORESIS, 6
(1975)
A comparative study of some kinetic and spectral properties of guanidinated and native cytochrome
C. Costa, J. Moura, I. Moura, M. Liu, H. Peck, J. Legall, Yaning Wang, Boi Huynhs (1990)
Hexaheme Nitrite Reductase from Desulfovibrio desulfuricans
V. Massey, P. Hemmerich, W. Knappe, H. Duchstein, H. Fenner (1978)
Photoreduction of flavoproteins and other biological compounds catalyzed by deazaflavins.Biochemistry, 17 1
M. Liu, H. Peck (1981)
The isolation of a hexaheme cytochrome from Desulfovibrio desulfuricans and its identification as a new type of nitrite reductase.The Journal of biological chemistry, 256 24
P. Smith, R. Krohn, G. Hermanson, A. Mallia, F. Gartner, M. Provenzano, E. Fujimoto, N. Goeke, B. Olson, D. Klenk (1985)
Measurement of protein using bicinchoninic acid.Analytical biochemistry, 150 1
T. Kakutani, H. Watanabe, K. Arima, T. Beppu (1981)
Purification and properties of a copper-containing nitrite reductase from a denitrifying bacterium, Alcaligenes faecalis strain S-6.Journal of biochemistry, 89 2
R. Blackmore, T. Brittain, P. Gadsby, C. Greenwood, A. Thomson (1987)
Electron paramagnetic resonance and magnetic circular dichroism studies of a hexa‐heme nitrite reductase from Wolinella succinogenesFEBS Letters, 219
(1991)
Investi - gations on microbial sulfur respiration . 2 . Isolation , purification , and characterization of cellular components from Spiril - lure 5175
(1988)
Ecology of denitrification and dissimilatory nitrate reduction to ammonium
M. Kennedy, H. Beinert, P. Kroneck (1988)
1. Activation and reduction of elemental sulfur in several strains of eubacteria
M. Liu, B. Bakel, M. Liu, T. Dao (1988)
Purification of Vibrio fischeri nitrite reductase and its characterization as a hexaheme c-type cytochrome.Archives of biochemistry and biophysics, 262 1
M.‐C. Liu, M.-Y. Liu, W. Payne, H. Peck, J. Gall, D. Dervartanian (1987)
Comparative EPR studies on the nitrite reductases from Escherichia coli and Wolinella succinogenesFEBS Letters, 218
R. Blackmore, A. Roberton, T. Brittain (1986)
The purification and some equilibrium properties of the nitrite reductase of the bacterium Wolinella succinogenes.The Biochemical journal, 233 2
W. Schroeder, J. Shelton, J. Shelton, B. Robberson, G. Apell, R. Fang, J. Bonaventura (1982)
The complete amino acid sequence of bovine liver catalase and the partial sequence of bovine erythrocyte catalase.Archives of biochemistry and biophysics, 214 1
J. Sadana, B. Khan, I. Fry, R. Cammack (1986)
Electron paramagnetic resonance studies of heme c and its nitrosyl derivative in Vibrio (Achromobacter) fischeri nitrite reductase.Biochemistry and cell biology = Biochimie et biologie cellulaire, 64 5
L. Barton, J. Legall, J. Odom, H. Peck (1983)
Energy coupling to nitrite respiration in the sulfate-reducing bacterium Desulfovibrio gigasJournal of Bacteriology, 153
R. Wolfe, N. Pfennig (1977)
Reduction of sulfur by spirillum 5175 and syntrophism with ChlorobiumApplied and Environmental Microbiology, 33
U. Laemmli (1970)
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 227
J. Gudat, Jasbir Singh, D. Wharton (1973)
Cytochrome oxidase from Pseudomonas aeruginosa. I. Purification and some properties.Biochimica et biophysica acta, 292 2
F. Widdel (1986)
Growth of Methanogenic Bacteria in Pure Culture with 2-Propanol and Other Alcohols as Hydrogen DonorsApplied and Environmental Microbiology, 51
J. Riester, W. Zumft, P. Kroneck (1989)
Nitrous oxide reductase from Pseudomonas stutzeri. Redox properties and spectroscopic characterization of different forms of the multicopper enzyme.European journal of biochemistry, 178 3
203 156 156 1 1 Wolfram Schumacher Peter M. H. Kroneck Fakultät Biologie Universität Konstanz W-7750 Konstanz Federal Republic of Germany Abstract When grown with nitrate as terminal electron acceptor both the soluble (periplasm, cytoplasm) and the membrane fraction of “Spirillum” strain 5175 exhibited high nitrite reductase activity. The nitrite reductase obtained from the soluble fraction was purified 76-fold to electrophoretical homogeneity. The enzyme reduced nitrite to ammonia with a specific activity of 723 μmol NO inf2 sup- × (mg protein × min) -1 . The molecular mass was 58±1 kDa by SDS-PAGE compared to 59±2 kDa determined by size exclusion chromatography under nondenaturing conditions. The enzyme (as isolated) contained 5.97±0.15 heme c molecules/M r 58 kDa. The absorption spectrum was typical for c -type cytochrome with maxima at 280, 408, 532 and 610 nm (oxidized) and at 420, 523 and 553 nm (dithionite-reduced). The enzyme (as isolated) exhibited a complex set of high-spin and lowspin ferric heme resonances with g-values at 9.82, 3,85, 3.31, 2.95, 2.30 and 1.49 in agreement with data reported for electron paramagnetic resonance spectra of nitrite reductases from Desulfovibrio desulfuricans, Wolinella succinogenes and Escherichia coli .
Archives of Microbiology – Springer Journals
Published: Jul 1, 1991
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.