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Determination of cell wall teichoic acid structure of staphylococci by rapid chemical and serological screening methods

Determination of cell wall teichoic acid structure of staphylococci by rapid chemical and... 203 137 137 3 3 J. Endl P. H. Seidl F. Fiedler K. H. Schleifer Lehrstuhl für Mikrobiologie Technische Universität München Arcisstr. 21 D-8000 München 2 Germany Lehrstuhl für Mikrobiologie Universität München Maria-Ward-Str. 1a D-8000 München 19 Germany Abstract Investigations of cell wall teichoic acid structures of various staphylococci were carried out by a rapid method based on the gas-liquid chromatographic separation of products obtained after treatment of phenol-extracted cells with 70% hydrofluoric acid. In most of the strains teichoic acids of the poly(glycerolphosphate) and/or poly(ribitolphosphate) type were found. Teichoic acids of the poly(glycerolphosphate-N-acetylglucosaminephosphate) type and polymers consisting of N-acetylglucosaminephosphate were present in few strains. The results obtained by the rapid chemical screening method were compared with data obtained by serological analysis of teichoic acid structures using specific antisera and the lectin wheat germ agglutinin. Teichoic acid components occurring in low concentrations could only be detected with the chemical and not with the serological method. A number of strains of species of the genus Staphylococcus have been studied using these rapid methods. With a few exceptions, the teichoic acid structure proved to be a constant marker within a given species. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Microbiology Springer Journals

Determination of cell wall teichoic acid structure of staphylococci by rapid chemical and serological screening methods

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References (15)

Publisher
Springer Journals
Copyright
Copyright © 1984 by Springer-Verlag
Subject
Life Sciences; Biotechnology; Biochemistry, general; Cell Biology; Ecology; Microbial Ecology; Microbiology
ISSN
0302-8933
eISSN
1432-072X
DOI
10.1007/BF00414557
Publisher site
See Article on Publisher Site

Abstract

203 137 137 3 3 J. Endl P. H. Seidl F. Fiedler K. H. Schleifer Lehrstuhl für Mikrobiologie Technische Universität München Arcisstr. 21 D-8000 München 2 Germany Lehrstuhl für Mikrobiologie Universität München Maria-Ward-Str. 1a D-8000 München 19 Germany Abstract Investigations of cell wall teichoic acid structures of various staphylococci were carried out by a rapid method based on the gas-liquid chromatographic separation of products obtained after treatment of phenol-extracted cells with 70% hydrofluoric acid. In most of the strains teichoic acids of the poly(glycerolphosphate) and/or poly(ribitolphosphate) type were found. Teichoic acids of the poly(glycerolphosphate-N-acetylglucosaminephosphate) type and polymers consisting of N-acetylglucosaminephosphate were present in few strains. The results obtained by the rapid chemical screening method were compared with data obtained by serological analysis of teichoic acid structures using specific antisera and the lectin wheat germ agglutinin. Teichoic acid components occurring in low concentrations could only be detected with the chemical and not with the serological method. A number of strains of species of the genus Staphylococcus have been studied using these rapid methods. With a few exceptions, the teichoic acid structure proved to be a constant marker within a given species.

Journal

Archives of MicrobiologySpringer Journals

Published: Mar 1, 1984

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