Access the full text.
Sign up today, get DeepDyve free for 14 days.
L. Brauns, M. Hudson, J. Oliver (1991)
Use of the polymerase chain reaction in detection of culturable and nonculturable Vibrio vulnificus cellsApplied and Environmental Microbiology, 57
A. Reinhartz, S. Alajem, A. Samson, M. Herzberg (1993)
A novel rapid hybridization technique: paper chromatography hybridization assay (PACHA).Gene, 136 1-2
H. Taniguchi, H. Hirano, S. Kubomura, K. Higashi, Y. Mizuguchi (1986)
Comparison of the nucleotide sequences of the genes for the thermostable direct hemolysin and the thermolabile hemolysin from Vibrio parahaemolyticus.Microbial pathogenesis, 1 5
R. Jefferson, S. Burgess, D. Hirsh (1986)
beta-Glucuronidase from Escherichia coli as a gene-fusion marker.Proceedings of the National Academy of Sciences of the United States of America, 83 22
C. Lee, S. Pan (1993)
Rapid and specific detection of the thermostable direct haemolysin gene in Vibrio parahaemolyticus by the polymerase chain reaction.Journal of general microbiology, 139 12
S. Pillai, K. Josephson, R. Bailey, C. Gerba, I. Pepper (1991)
Rapid method for processing soil samples for polymerase chain reaction amplification of specific gene sequencesApplied and Environmental Microbiology, 57
Sidney Suggs, T. Hirose, T. Miyake, Eric Kawashima, M. Johnson, K. Itakura, R. Wallace (1981)
USE OF SYNTHETIC OLIGODEOXYRIBONUCLEOTIDES FOR THE ISOLATION OF SPECIFIC CLONED DNA SEQUENCES1
Daniel Jones, R. Law, A. Bej (1993)
Detection of Salmonella spp. in Oysters Using Polymerase Chain Reactions (PCR) and Gene ProbesJournal of Food Science, 58
R. Saiki, D. Gelfand, S. Stoffel, S. Scharf, R. Higuchi, G. Horn, K. Mullis, H. Erlich (1988)
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.Science, 239 4839
J. Tada, T. Ohashi, N. Nishimura, Y. Shirasaki, Hiroko Ozaki, Shigeru Fukushima, Jun Takano, M. Nishibuchi, Y. Takeda (1992)
Detection of the thermostable direct hemolysin gene (tdh) and the thermostable direct hemolysin-related hemolysin gene (trh) of Vibrio parahaemolyticus by polymerase chain reaction.Molecular and cellular probes, 6 6
K. Mullis (1990)
The unusual origin of the polymerase chain reaction.Scientific American, 262 4
K. Klontz, S. Lieb, M. Schreiber, H. Janowski, L. Baldy, R. Gunn (1988)
Syndromes of Vibrio vulnificus infections. Clinical and epidemiologic features in Florida cases, 1981-1987.Annals of internal medicine, 109 4
A. Bej, Wee-Yao Ng, Shellie Morgan, Daniel Jones, M. Mahbubani (1996)
Detection of viableVibrio cholerae by reverse-transcriptase polymerase chain reaction (RT-PCR)Molecular Biotechnology, 5
J. Mekalanos, Daryl Swartz, G. Pearson, N. Harford, Francoise Groyne, M. Wilde (1983)
Cholera toxin genes: nucleotide sequence, deletion analysis and vaccine developmentNature, 306
Jeffrey Miller (1972)
Experiments in molecular genetics
A. Bosch, F. Abad, R. Gajardo, R. Pintó (1994)
Should shellfish be purified before public consumption?The Lancet, 344
J. Galán, C. Ginocchio, P. Costeas (1992)
Molecular and functional characterization of the Salmonella invasion gene invA: homology of InvA to members of a new protein familyJournal of Bacteriology, 174
Multiplex PCR amplification of uidA, cth, invA, ctx, and tl genes was developed enabling simultaneous detection in shellfish of Escherichia coli, an indicator of fecal contamination and microbial pathogens, Salmonella typhimurium, Vibrio vulnificus, V. cholerae, and V. parahaemolyticus, respectively. Each of the five pairs of oligonucleotide primers was found to support PCR amplifications of only its targeted gene. The optimized multiplex PCR reaction utilized a PCR reaction buffer containing 2.5 mM MgCl2 and primer annealing temperature of 55°C. Oyster tissue homogenate seeded with these microbial pathogens was subjected to DNA purification by the Chelex™ 100 (BioRad) method. The sensitivity of detection for each of the microbial pathogens was ≤101–102 cells following a “double” multiplex PCR amplification approach. Amplified target genes in a multiplex PCR reaction were subjected to a colorimetric GeneComb™ (BioRad) DNA-DNA hybridization assay. This assay was rapid and showed sensitivity of detection comparable to the agarose gel electrophoresis method. The colorimetric GeneComb™ assay avoids use of hazardous materials inherent in conventional gel electrophoresis and radioactive-based hybridization methods. Multiplex PCR amplification, followed by colorimetric GeneComb™ DNA-DNA hybridization, has been shown to be an effective, sensitive, and rapid method to detect microbial pathogens in shellfish.
Current Microbiology – Springer Journals
Published: Aug 1, 1998
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.