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Construction of a human heart cDNA library and identification of cardiovascular based genes (CVBest)

Construction of a human heart cDNA library and identification of cardiovascular based genes (CVBest) The availability of high quality cDNA libraries is often crucial to the successful identification and characterization of genes. The concepts and potential pitfalls of constructing cDNA libraries are presented. Various applications requiring high quality cDNA libraries are outlined, including large-scale single pass sequencing of cDNA clones to generate expressed sequence tags (ESTs) and differential screening of cDNA libraries. The usefulness of combining such approaches for the discovery of novel disease-related and cardiovascular-based ESTs (CVBest) is discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular and Cellular Biochemistry Springer Journals

Construction of a human heart cDNA library and identification of cardiovascular based genes (CVBest)

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References (22)

Publisher
Springer Journals
Copyright
Copyright © 1997 by Kluwer Academic Publishers
Subject
Life Sciences; Cardiology; Medical Biochemistry; Oncology; Biochemistry, general
ISSN
0300-8177
eISSN
1573-4919
DOI
10.1023/A:1006811403996
Publisher site
See Article on Publisher Site

Abstract

The availability of high quality cDNA libraries is often crucial to the successful identification and characterization of genes. The concepts and potential pitfalls of constructing cDNA libraries are presented. Various applications requiring high quality cDNA libraries are outlined, including large-scale single pass sequencing of cDNA clones to generate expressed sequence tags (ESTs) and differential screening of cDNA libraries. The usefulness of combining such approaches for the discovery of novel disease-related and cardiovascular-based ESTs (CVBest) is discussed.

Journal

Molecular and Cellular BiochemistrySpringer Journals

Published: Sep 20, 2004

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