Access the full text.
Sign up today, get DeepDyve free for 14 days.
D. Hwang, Y. Fung, R. Wang, C. Laurenssen, S. Ng, W. Lam, K. Tsui, K. Fung, M. Waye, C. Lee (1995)
Analysis of expressed sequence tags from a fetal human heart cDNA library.Genomics, 30 2
J. Kelley, J. Gocayne, M. Dubnick, Hong Xiao, Carl Merril, Andrew Wu, Bjorn Olde, Ruben Moreno, A. Kerlavage, W. McCombie (1991)
Complementary DNA sequencing: expressed sequence tags and human genome projectScience, 252
(1987)
One-tube doublestranded cDNA synthesis using cloned m-mlv reverse transcriptase
S. Altschul, W. Gish, W. Miller, E. Myers, D. Lipman (1990)
Basic local alignment search tool.Journal of molecular biology, 215 3
K. Sudo, K. Chinen, Y. Nakamura (1994)
2058 expressed sequence tags (ESTs) from a human fetal lung cDNA library.Genomics, 24 2
C. Liew, D. Hwang, Y. Fung, C. Laurenssen, E. Cukerman, S. Tsui, C. Lee (1994)
A catalogue of genes in the cardiovascular system as identified by expressed sequence tags.Proceedings of the National Academy of Sciences of the United States of America, 91 22
David Hwang, Wei-Sek Hwang, Choong-Chin Liew (1994)
Single pass sequencing of a unidirectional human fetal heart cDNA library to discover novel genes of the cardiovascular system.Journal of molecular and cellular cardiology, 26 10
Y. Fung, C. Liew (1996)
Identification of genes associated with myocardial development.Journal of molecular and cellular cardiology, 28 6
M. Adams, M. Soares, A. Kerlavage, C. Fields, J. Venter (1993)
Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA libraryNature Genetics, 4
C. Liew (1993)
A human heart cDNA library--the development of an efficient and simple method for automated DNA sequencing.Journal of molecular and cellular cardiology, 25 8
(1995)
Analysis of expressed sequence tags from a fetal human heart
M. Adams, A. Kerlavage, C. Fields, J. Venter (1993)
3,400 new expressed sequence tags identify diversity of transcripts in human brainNature Genetics, 4
W. Gish, D. States (1993)
Identification of protein coding regions by database similarity searchNature Genetics, 3
U. Gubler, B. Hoffman (1983)
A simple and very efficient method for generating cDNA libraries.Gene, 25 2-3
P. Chomczyński, N. Sacchi (1987)
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.Analytical biochemistry, 162 1
Akbar Khan, A. Wilcox, M. Polymeropoulos, J. Hopkins, T. Stevens, M. Robinson, A. Orpana, J. Sikela (1992)
Single pass sequencing and physical and genetic mapping of human brain cDNAsNature Genetics, 2
J. Frigerio, P. Berthézène, P. Garrido, E. Ortiz, S. Barthellémy, S. Vasseur, B. Sastre, I. Seleznieff, J. Dagorn, J. Iovanna (1995)
Analysis of 2166 clones from a human colorectal cancer cDNA library by partial sequencing.Human molecular genetics, 4 1
(1992)
Expressed sequence tags and chromosomal localization of cDNA clones from a subtracted retinal pigment epithelium
J. Takeda, H. Yano, S. Eng, Y. Zeng, G. Bell (1993)
A molecular inventory of human pancreatic islets: sequence analysis of 1000 cDNA clones.Human molecular genetics, 2 11
L. Gieser, A. Swaroop (1992)
Expressed sequence tags and chromosomal localization of cDNA clones from a subtracted retinal pigment epithelium library.Genomics, 13 3
K. Okubo, N. Hori, R. Matoba, T. Niiyama, A. Fukushima, Y. Kojima, K. Matsubara (1992)
Large scale cDNA sequencing for analysis of quantitative and qualitative aspects of gene expressionNature Genetics, 2
M. Adams, M. Dubnick, A. Kerlavage, R. Moreno, J. Kelley, T. Utterback, James Nagle, C. Fields, J. Venter (1992)
Sequence identification of 2,375 human brain genesNature, 355
The availability of high quality cDNA libraries is often crucial to the successful identification and characterization of genes. The concepts and potential pitfalls of constructing cDNA libraries are presented. Various applications requiring high quality cDNA libraries are outlined, including large-scale single pass sequencing of cDNA clones to generate expressed sequence tags (ESTs) and differential screening of cDNA libraries. The usefulness of combining such approaches for the discovery of novel disease-related and cardiovascular-based ESTs (CVBest) is discussed.
Molecular and Cellular Biochemistry – Springer Journals
Published: Sep 20, 2004
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.