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Conservation of a highly repeated DNA family of Aedes albopictus among mosquito genomes (Diptera: Culicidae)

Conservation of a highly repeated DNA family of Aedes albopictus among mosquito genomes (Diptera:... 122 83 83 5 5 A. Kumar K. S. Rai Department of Biological Sciences University of Notre Dame 46556 Notre Dame IN USA Summary The genomic organization and chromosomal localization of a cloned 0.79-kb highly repeated DNA fragment, H-115, isolated from Aedes albopictus has been examined. The cloned fragment is a part of a larger unit of 1.86 kb that is tandemly repeated in the Ae. albopictus genome. The H-115 family of sequences are located at the intercalary position on chromosome 1 in Ae. albopictus . Similar patterns of in situ and Southern blot hybridization results are obtained in Ae. aegypti, Ae. seatoi, Ae. flavopictus, Ae. polynesiensis, Ae. Alcasidi , and Ae. katherinensis . The H-115 sequences are widely conserved in Culicidae and are found in Haemagogus equinus, Tripteroides bambusa , and Anopheles quadrimaculatus by hybridization under high stringency conditions. The H-115 sequences are also tandemly repeated in Hg. equinus with a monomer unit of 1.86 kb and in Tp. bambusa with a slightly diverged monomer unit of 1.90kb. In Anopheles quadrimaculatus , the H-115 sequences are dispersed throughout the genome. Partial sequence analysis shows that the H-115 insert is 62% AT and contains two perfect inverted repeats and numerous perfect direct repeats. The occurrence of inverted repeats with potential to form intrastrand palindromic structure suggests that the H-115 family of sequences may be involved in chromatin condensation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png TAG Theoretical and Applied Genetics Springer Journals

Conservation of a highly repeated DNA family of Aedes albopictus among mosquito genomes (Diptera: Culicidae)

Kumar, A.; Rai, K.
TAG Theoretical and Applied Genetics , Volume 83 (5) – Mar 1, 1992
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References (36)

Publisher
Springer Journals
Copyright
Copyright © 1995 by Springer-Verlag
Subject
Life Sciences; Biotechnology; Agriculture; Biochemistry, general; Plant Biochemistry; Plant Sciences; Plant Genetics & Genomics
ISSN
0040-5752
eISSN
1432-2242
DOI
10.1007/BF00226899
pmid
24202672
Publisher site
See Article on Publisher Site

Abstract

122 83 83 5 5 A. Kumar K. S. Rai Department of Biological Sciences University of Notre Dame 46556 Notre Dame IN USA Summary The genomic organization and chromosomal localization of a cloned 0.79-kb highly repeated DNA fragment, H-115, isolated from Aedes albopictus has been examined. The cloned fragment is a part of a larger unit of 1.86 kb that is tandemly repeated in the Ae. albopictus genome. The H-115 family of sequences are located at the intercalary position on chromosome 1 in Ae. albopictus . Similar patterns of in situ and Southern blot hybridization results are obtained in Ae. aegypti, Ae. seatoi, Ae. flavopictus, Ae. polynesiensis, Ae. Alcasidi , and Ae. katherinensis . The H-115 sequences are widely conserved in Culicidae and are found in Haemagogus equinus, Tripteroides bambusa , and Anopheles quadrimaculatus by hybridization under high stringency conditions. The H-115 sequences are also tandemly repeated in Hg. equinus with a monomer unit of 1.86 kb and in Tp. bambusa with a slightly diverged monomer unit of 1.90kb. In Anopheles quadrimaculatus , the H-115 sequences are dispersed throughout the genome. Partial sequence analysis shows that the H-115 insert is 62% AT and contains two perfect inverted repeats and numerous perfect direct repeats. The occurrence of inverted repeats with potential to form intrastrand palindromic structure suggests that the H-115 family of sequences may be involved in chromatin condensation.

Journal

TAG Theoretical and Applied Genetics – Springer Journals

Published: Mar 1, 1992

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