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Chromate resistance and reduction in Pseudomonas fluorescens strain LB300

Chromate resistance and reduction in Pseudomonas fluorescens strain LB300 203 150 150 5 5 Lawrence H. Bopp Henry L. Ehrlich Department of Biology Rensselaer Polytechnic Institute 12180-3590 Troy NY USA New York State Department of Health WCLR, Empire State Plaza 12201 Albany NY USA Abstract Pseudomonas fluorescens LB300 is a chromateresistant strain isolated from chromium-contaminated river sediment. Chromate resistance is conferred by the plasmid pLHB1. Strain LB300 grew in minimal salts medium with as much as 1000 μg of K 2 CrO 4 ml −1 , and actively reduced chromate to Cr(III) while growing aerobically on a variety of substrates. Chromate was also reduced during anaerobic growth on acetate, the chromate serving as terminal electron acceptor. P. fluorescens LB303, a plasmidless, chromatesensitive variant of P. fluorescens LB300, did not grow in minimal salts medium with more than 10 μg of K 2 CrO 4 ml −1 . However, resting cells of strain LB303 grown without chromate reduced chromate as well as strain LB300 cells grown under the same conditions. Furthermore, resting cells of chromate-sensitive Pseudomonas putida strain AC10, also catalyzed chromate reduction. Evidently chromate resistance and chromate reduction in these organisms are unrelated. Comparison of the rates of chromate reduction by chromate grown cells and cells grown without chromate indicated that the chromate reductase activity is constitutive. Studies with cell-free extracts show that the reductase is membrane-associated and can mediate the transfer of electrons from NADH to chromate. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Microbiology Springer Journals

Chromate resistance and reduction in Pseudomonas fluorescens strain LB300

Bopp, Lawrence; Ehrlich, Henry
Archives of Microbiology , Volume 150 (5) – Sep 1, 1988
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References (19)

Publisher
Springer Journals
Copyright
Copyright © 1988 by Springer-Verlag
Subject
Life Sciences; Biotechnology; Biochemistry, general; Cell Biology; Ecology; Microbial Ecology; Microbiology
ISSN
0302-8933
eISSN
1432-072X
DOI
10.1007/BF00422281
Publisher site
See Article on Publisher Site

Abstract

203 150 150 5 5 Lawrence H. Bopp Henry L. Ehrlich Department of Biology Rensselaer Polytechnic Institute 12180-3590 Troy NY USA New York State Department of Health WCLR, Empire State Plaza 12201 Albany NY USA Abstract Pseudomonas fluorescens LB300 is a chromateresistant strain isolated from chromium-contaminated river sediment. Chromate resistance is conferred by the plasmid pLHB1. Strain LB300 grew in minimal salts medium with as much as 1000 μg of K 2 CrO 4 ml −1 , and actively reduced chromate to Cr(III) while growing aerobically on a variety of substrates. Chromate was also reduced during anaerobic growth on acetate, the chromate serving as terminal electron acceptor. P. fluorescens LB303, a plasmidless, chromatesensitive variant of P. fluorescens LB300, did not grow in minimal salts medium with more than 10 μg of K 2 CrO 4 ml −1 . However, resting cells of strain LB303 grown without chromate reduced chromate as well as strain LB300 cells grown under the same conditions. Furthermore, resting cells of chromate-sensitive Pseudomonas putida strain AC10, also catalyzed chromate reduction. Evidently chromate resistance and chromate reduction in these organisms are unrelated. Comparison of the rates of chromate reduction by chromate grown cells and cells grown without chromate indicated that the chromate reductase activity is constitutive. Studies with cell-free extracts show that the reductase is membrane-associated and can mediate the transfer of electrons from NADH to chromate.

Journal

Archives of Microbiology – Springer Journals

Published: Sep 1, 1988

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