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Journals Lipids Volume 41 Issue 11

The scavenger receptor class B, type I (SR-BI) is an important player in regulation of mammalian lipid homeostasis. We therefore wanted to study this receptor in Atlantic salmon ( Salmo salar L.), which requires a diet with particular high lipid content. We have for the first time cloned and characterized SR-BI from a salmonid fish. The predicted 494 amino acid protein contained two transmembrane domains, several putative N-glycosylation sites, and showed 72% sequence identity with the predicted homolog from zebrafish. SR-BI expression was analyzed by reverse transcription Real-Time PCR in several tissues, and a high relative expression in salmon midgut was detected, which may suggest that SR-BI has a role in uptake of lipids from the diet. We also expressed a construct of salmon myc -tagged SR-BI in salmon TO cells and HeLa cells, which gave a protein of approximately 80 kDa on reducing SDS-PAGE using an antibody against the myc -epitope. Immunofluorescence microscopy analyses of the salmon SR-BI protein in transiently transfected HeLa cells revealed staining in the cell periphery and in some intracellular membranes, but not in the nucleus, which indicated that the salmon protein may be a functional membrane protein. We also observed a high degree of co-localization using an anti-peptide SR-BI antiserum. We found that 20 μg mL −1 insulin up-regulated the SR-BI mRNA levels in primary cultures of salmon hepatocytes relative to untreated cells. Oleic acid, EPA, DHA, or dexamethasone did not affect the relative expression of SR-BI in this liver model system. In conclusion, the salmon SR-BI cDNA encoded a protein with several features common to those of mammalian species. SR-BI gene expression was high in the intestine, which leads us to propose that SR-BI may contribute to the uptake of lipids from the diet.

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Characterization of scavenger receptor class B, type I in Atlantic salmon ( Salmo salar L.)

E., Kleveland; B., Syvertsen; B., Ruyter; A., Vegusdal; S., Jørgensen; T., Gjøen
Lipids , Volume 41 (11)
Springer Journals – Nov 1, 2006

More Info
  • Publisher Springer-Verlag
  • Copyright Copyright © 2006 by AOCS Press
  • Subject Life Sciences; Nutrition; Bioorganic Chemistry; Medicinal Chemistry; Medical Biochemistry; Biochemistry, general; Microbial Genetics and Genomics
  • ISSN 0024-4201
  • eISSN 1558-9307
  • D.O.I. 10.1007/s11745-006-5052-3
  • Publisher site Get PDF  

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