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Characterization of deletions in common wheat induced by an Aegilops cylindrica chromosome: detection of multiple chromosome rearrangements

Characterization of deletions in common wheat induced by an Aegilops cylindrica chromosome:... 122 91 91 4 4 U. Hohmann T. R. Endo R. G. Herrmann B. S. Gill Botanisches Institut der Ludwig-Maximilians-Universität München Menzinger Strasse 67 D-80638 Munich Germany Laboratory of Genetics, Faculty of Agriculture Kyoto University 606 Kyoto Japan Department of Plant Pathology Kansas State University 66506 Manhattan KS USA Abstract An Aegilops cylindrica chromosome induces terminal deletions of chromosomes in wheat as identified by C-banding. We are constructing high-density physical maps of wheat chromosomes and have detected additional chromosome rearrangements. Among 63 lines with chromosomal subarm deletions in group 7 chromosomes, 7 lines (11.1%) were shown to harbor additional chromosome rearrangements. Two other lines were also omitted from the physical mapping because of the nature of the breakpoint calculations. The presence or absence of chromosome-specific restriction fragment length polymorphism (RFLP) or random amplified polymorphic DNA (RAPD) markers indicated that additional interstitial deletions are present in 3 lines (4.8%) with deletions in the short chromosome arms and in 4 lines (6.3%) with deletions in the long chromosome arms. We also used chromosome pairing analysis of F 1 plants of deletion lines with double ditelosomic lines of ‘Chinese Spring’ wheat to detect small terminal deletions. The deletion of the most distal 1% of chromosome arm 7AL was associated with a pairing reduction of 60%. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png TAG Theoretical and Applied Genetics Springer Journals

Characterization of deletions in common wheat induced by an Aegilops cylindrica chromosome: detection of multiple chromosome rearrangements

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References (25)

Publisher
Springer Journals
Copyright
Copyright © 1995 by Springer-Verlag
Subject
Life Sciences; Biotechnology; Agriculture; Biochemistry, general; Plant Biochemistry; Plant Sciences; Plant Genetics & Genomics
ISSN
0040-5752
eISSN
1432-2242
DOI
10.1007/BF00223287
pmid
24169888
Publisher site
See Article on Publisher Site

Abstract

122 91 91 4 4 U. Hohmann T. R. Endo R. G. Herrmann B. S. Gill Botanisches Institut der Ludwig-Maximilians-Universität München Menzinger Strasse 67 D-80638 Munich Germany Laboratory of Genetics, Faculty of Agriculture Kyoto University 606 Kyoto Japan Department of Plant Pathology Kansas State University 66506 Manhattan KS USA Abstract An Aegilops cylindrica chromosome induces terminal deletions of chromosomes in wheat as identified by C-banding. We are constructing high-density physical maps of wheat chromosomes and have detected additional chromosome rearrangements. Among 63 lines with chromosomal subarm deletions in group 7 chromosomes, 7 lines (11.1%) were shown to harbor additional chromosome rearrangements. Two other lines were also omitted from the physical mapping because of the nature of the breakpoint calculations. The presence or absence of chromosome-specific restriction fragment length polymorphism (RFLP) or random amplified polymorphic DNA (RAPD) markers indicated that additional interstitial deletions are present in 3 lines (4.8%) with deletions in the short chromosome arms and in 4 lines (6.3%) with deletions in the long chromosome arms. We also used chromosome pairing analysis of F 1 plants of deletion lines with double ditelosomic lines of ‘Chinese Spring’ wheat to detect small terminal deletions. The deletion of the most distal 1% of chromosome arm 7AL was associated with a pairing reduction of 60%.

Journal

TAG Theoretical and Applied Genetics – Springer Journals

Published: Sep 1, 1995

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