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A. Jeltsch, T. Lanio (2002)
Site-directed mutagenesis by polymerase chain reaction.Methods in molecular biology, 182
S. Kunugi, N. Tanaka (2002)
Cold denaturation of proteins under high pressure.Biochimica et biophysica acta, 1595 1-2
M. Meng, C. Lee, M. Bagdasarian, J. Zeikus (1991)
Switching substrate preference of thermophilic xylose isomerase from D-xylose to D-glucose by redesigning the substrate binding pocket.Proceedings of the National Academy of Sciences of the United States of America, 88
Y. Bor, Celia Moraes, Sam-Pin Lee, William Crosby, A. Sinskey, C. Batt (1992)
Cloning and sequencing the Lactobacillus brevis gene encoding xylose isomerase.Gene, 114 1
Zacharias Dische, Ellen Borenfreund (1951)
A new spectrophotometric method for the detection and determination of keto sugars and trioses.The Journal of biological chemistry, 192 2
(1993)
cloning , sequencing and biochemical characterization of xylose isomerase from Thermoanaerobacterium saccharolyticum strain B 6 a
R. Amore, C. Hollenberg (1989)
Xylose isomerase from Actinoplanes missouriensis: primary structure of the gene and the protein.Nucleic acids research, 17 18
P. Meaden, J. Aduse-Opoku, J. Reizer, A. Reizer, Y. Lanceman, M. Martin, W. Mitchell (1994)
The xylose isomerase-encoding gene (xylA) of Clostridium thermosaccharolyticum: cloning, sequencing and phylogeny of XylA enzymes.Gene, 141 1
(1951)
a new spectrophotometric method for the detection and determination of keto sugars and tri - oses
(2002)
site-directed mutagenesis by polymerase chain reaction, vol 182 In vitro mutagenesis protocols
Hespell rB (1992) fermentation of xylans by Butyrivibrio fibrisolvens and Thermoanaerobacter strain B6a: utilization of uronic acids and xylanolytic activites
Snehalata Bhosale, M. Rao, V. Deshpande (1996)
Molecular and industrial aspects of glucose isomerase.Microbiological reviews, 60 2
Stephen Brown, C. Sjøholm, R. Kelly (1993)
Purification and characterization of a highly thermostable glucose isomerase produced by the extremely thermophilic eubacterium, Thermotoga maritimaBiotechnology and Bioengineering, 41
R. Antrim, W. Colilla, B. Schnyder (1979)
Glucose Isomerase Production of High-Fructose Syrups
Y. Lee, M. Ramesh, J. Zeikus (1993)
Cloning, sequencing and biochemical characterization of xylose isomerase from Thermoanaerobacterium saccharolyticum strain B6A-RI.Journal of general microbiology, 139 Pt 6
C. Lee, J. Zeikus (1991)
Purification and characterization of thermostable glucose isomerase from Clostridium thermosulfurogenes and Thermoanaerobacter strain B6A.The Biochemical journal, 273 ( Pt 3)
Rockey Bandlish, J. Hess, K. Epting, C. Vieille, R. Kelly (2002)
Glucose-to-fructose conversion at high temperatures with xylose (glucose) isomerases from Streptomyces murinus and two hyperthermophilic Thermotoga species.Biotechnology and bioengineering, 80 2
A. Converti, M. Borghi (1997)
Kinetics of glucose isomerization to fructose by immobilized glucose isomerase in the presence of substrate protectionBioprocess Engineering, 18
W. Mu, Xiaole Wang, Qinghai Xue, B. Jiang, Tao Zhang, M. Miao (2012)
Characterization of a thermostable glucose isomerase with an acidic pH optimum from Acidothermus cellulolyticusFood Research International, 47
L. Lama, B. Nicolaus, V. Calandrelli, I. Romano, R. Basile, A. Gambacorta (2001)
Purification and characterization of thermostable xylose(glucose) isomerase from Bacillus thermoantarcticusJournal of Industrial Microbiology and Biotechnology, 27
J. Braman (2010)
In Vitro Mutagenesis Protocols
(1991)
( xylA ) of Clostridium thermosaccharolyticum : cloning , sequencing and phylogeny of Xy 1 a enzymes
(1994)
the xylose isomerase - encoding
V. Jensen, S. Rugh (1987)
[33] Industrial-scale production and application of immobilized glucose isomeraseMethods in Enzymology, 136
P. Privalov (1990)
Cold Denaturation of ProteinCritical Reviews in Biochemistry and Molecular Biology, 25
K. Dekker, H. Yamagata, K. Sakaguchi, S. Udaka (1991)
Xylose (glucose) isomerase gene from the thermophile Clostridium thermohydrosulfuricum; cloning, sequencing, and expression in Escherichia coli.Agricultural and biological chemistry, 55 1
M. Borgi, M. Rhimi, S. Bejar (2007)
Involvement of alanine 103 residue in kinetic and physicochemical properties of glucose isomerases from Streptomyces species.Biotechnology journal, 2 2
Karima Srih-Belghith, S. Bejar (1998)
A thermostable glucose isomerase having a relatively low optimum pH: study of activity and molecular cloning of the corresponding geneBiotechnology Letters, 20
R. Hespell (1992)
Fermentation of xylans byButyrivibrio fibrisolvens andThermoanaerobacter strain B6A: Utilization of uronic acids and xylanolytic activitesCurrent Microbiology, 25
J. Cha, C. Batt (1998)
Lowering the pH optimum of D-xylose isomerase: the effect of mutations of the negatively charged residues.Molecules and cells, 8 4
(1998)
Batt c (1998) lowering the pH optimum of d-xylose isomerase: the effect of mutations of the negatively charged residues. Mol cells
M. Borgi, Karima Srih-Belguith, M. Ali, M. Mezghani, S. Tranier, R. Haser, S. Bejar (2004)
Glucose isomerase of the Streptomyces sp. SK strain: purification, sequence analysis and implication of alanine 103 residue in the enzyme thermostability and acidotolerance.Biochimie, 86 8
(2001)
Purification and characterization of thermosta - ble xylose ( glucose ) isomerase from Bacillus thermoantarcti
A series of site-directed mutant glucose isomerase at tryptophan 139 from Thermoanaerobacterium saccharolyticum strain B6A were purified to gel electrophoretic homogeneity, and the biochemical properties were determined. W139F mutation is the most efficient mutant derivative with a tenfold increase in its catalytic efficiency toward glucose compared with the native GI. With a maximal activity at 80 °C of 59.58 U/mg on glucose, this mutant derivative is the most active type ever reported. The enzyme activity was maximal at 90 °C and like other glucose isomerase, this mutant enzyme required Co2+ or Mg2+ for enzyme activity and thermal stability (stable for 20 h at 80 °C in the absence of substrate). Its optimum pH was around 7.0, and it had 86 % of its maximum activity at pH 6.0 incubated for 12 h at 60 °C. This enzyme was determined as thermostable and weak-acid stable. These findings indicated that the mutant GI W139F from T. saccharolyticum strain B6A is appropriate for use as a potential candidate for high-fructose corn syrup producing enzyme.
Journal of Industrial Microbiology Biotechnology – Springer Journals
Published: Aug 20, 2014
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